Glucose 6 Phosphate Dehydrogenase Antibody (2H7)

Images

 
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Western blot analysis using Glucose 6 Phosphate Dehydrogenase mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysate.
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb ...read more
Flow Cytometry: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Flow cytometric analysis of Jurkat cells using Glucose 6 Phosphate Dehydrogenase mouse mAb (green) and negative control (red).
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Western blot analysis using Glucose 6 Phosphate Dehydrogenase mAb against human Glucose 6 Phosphate Dehydrogenase (AA: 275-515) recombinant ...read more
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb ...read more
ELISA: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC-P
Clone
2H7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Glucose 6 Phosphate Dehydrogenase Antibody (2H7) Summary

Immunogen
Purified recombinant fragment of human Glucose 6 Phosphate Dehydrogenase expressed in E. coli. [UniProt# P11413]
Marker
Cytosol Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
G6PD
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This Glucose 6 Phosphate Dehydrogenase (2H7) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-22125 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Glucose 6 Phosphate Dehydrogenase Antibody (2H7)

  • G6PD
  • G6PD1EC 1.1.1.49
  • glucose-6-phosphate dehydrogenase

Background

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glucose 6 Phosphate Dehydrogenase Antibody (NBP2-22125) (0)

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Review for Glucose 6 Phosphate Dehydrogenase Antibody (NBP2-22125) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-22125:
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reviewed by:
Anonymous
WB Human 09/08/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol

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Video Protocols

WB Video Protocol

FAQs for Glucose 6 Phosphate Dehydrogenase Antibody (NBP2-22125) (0)

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Secondary Antibodies

 

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Anonymous
09/08/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol G6PD
Entrez
Uniprot