Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysates.

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of paraffin-embedded kidney cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb with DAB staining.

Flow Cytometry: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of Jurkat cells using Glucose 6 Phosphate Dehydrogenase mouse mAb (green) and negative control (red).

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mAb against human Glucose 6 Phosphate Dehydrogenase (AA: 275-515) recombinant protein. (Expected MW is 53.1 kDa)

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of paraffin-embedded breast cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb with DAB staining.

ELISA: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).

A431 human skin carcinoma cell line was stained with Mouse anti-Glucose 6 Phosphate Dehydrogenase Protein-G purified Monoclonal Antibody conjugated to Phycoerythrin (Catalog # NBP2-22125PE, blue histogram) or matched control antibody (Catalog # NBP2-27231PE, orange histogram).

Simple Western: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125] - B[a]P-Induced Changes in Cell Cycle. (a) Beas-2B cells treated with 1 µM or 10 µM B[a]P (24 h), 3 µg/mL aphidicolin (18 h), & 100 ng/mL nocodazole (18 h) were prepared for cell imaging post-treatment & immunofluorescent staining. A reference cell cycle diagram is shown. The categorization schematic applied to immunofluorescent images is presented as well as a reference diagram of mammalian cell cycle. Scale bar = 25 μm. (b) Beas-2B cells treated with B[a]P were assessed for WST1 metabolism. n = 5. (c) Quantitative EdU uptake among EdU + Beas-2B cells treated with B[a]P. n = 4. (d) Cumulative cell cycle phase distributions of Beas-2B cells treated with B[a]P or cell cycle controls are presented. n = 4, except nocodazole-treated Beas-2B for which n = 2. (e) Magnitude changes compared to untreated controls among each categorical cell cycle phase are presented; bar colors coincide with phase designations presented in (d). n = 4, except nocodazole-treated Beas-2B for which n = 2. Positive changes denote increased percentage of cells in a specific phase among treated cells, whilst negative values denote decreased percentage. Endpoints are the arithmetic mean of listed independent experiments with error bars denoting one standard error of the mean. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37217524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA, Flow, IHC
• Clone: 2H7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free Summary

• Description: Novus Biologicals Mouse Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free (NBP2-22125) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and Simple Western. Anti-Glucose 6 Phosphate Dehydrogenase Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Glucose 6 Phosphate Dehydrogenase expressed in E. coli. [Uniprot: P11413]
• Marker: Cytosol Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: G6PD
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Simple Western 1:50
  • Western Blot 1:500-1:1000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free

• G6PD
• G6PD1EC 1.1.1.49
• G6PDH
• glucose-6-phosphate dehydrogenase

Background

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glucose 6 Phosphate Dehydrogenase Antibody (NBP2-22125)(3)

Publications using NBP2-22125

• Coyle J, Johnson C, Jensen J et al. Variation in Pentose Phosphate Pathway-associated Metabolism Dictates Cytotoxicity Outcomes Determined by Tetrazolium Reduction Assays Research Square 2023-02-13 [PMID: 37217524] (Simple Western, Human)
• Mele L, la Noce M, Paino F, et al. Glucose-6-phosphate dehydrogenase blockade potentiates tyrosine kinase inhibitor effect on breast cancer cells through autophagy perturbation. J Exp Clin Cancer Res. 2019-04-12 [PMID: 30987650] (WB, Human)
• Mele L, Paino F, Papaccio F, et al. A new inhibitor of glucose-6-phosphate dehydrogenase blocks pentose phosphate pathway and suppresses malignant proliferation and metastasis in vivo. Cell Death Dis. 2018-05-14 [PMID: 29760380] (WB, Human)

Review for Glucose 6 Phosphate Dehydrogenase Antibody (NBP2-22125) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 09/08/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell
• Species: Human

Bioinformatics

• Gene Symbol: G6PD
• Entrez:
  • 2539(Human)
• Uniprot:
  • P11413()