GLI-2 Antibody

Images

 
Western Blot: GLI-2 Antibody [NB600-874] - Analysis of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains ...read more
Immunohistochemistry-Paraffin: GLI-2 Antibody [NB600-874] - Mouse adenocarcinoma section shows high and diffuse nuclear Gli2 expression. IHC-P image submitted by a verified customer review.
Flow (Intracellular): GLI-2 Antibody [NB600-874] - intracellular staining for GLI-2 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-2 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) ...read more
Immunohistochemistry: GLI-2 Antibody [NB600-874] - Staining of tumor cells in human breast tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.
Affinity Purified anti-Gli2 antibody shows strong cytoplasmic and membranous staining of tumor cells in human breast tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, ...read more
Western blot using affinity purified antibody shows detection of Gli-2 protein. Lane 1: rat testes

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GLI-2 Antibody Summary

Description
This affinity-purified antibody is directed against human Gli-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with GLI-2 from human and chimpanzee based on the immunizing sequence.

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
This affinity purified GLI-2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human GLI-2 (isoform a). (Uniprot: P10070)
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLI2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • ELISA 1:10000-1:30000
  • Flow (Intracellular)
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:2000
Application Notes
This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot. Multiple splice variants have been reported for this protein a, b, g and d (133.3, 131.6, 88.1 and 86.4 kDa respectively). Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Use in chromatin immunoprecipitation reported in scientific literature (PMID: 27760825).
Reviewed Applications
Read 1 Review rated 5
using
NB600-874 in the following applications:

Publications
Read Publications using
NB600-874 in the following applications:

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with Gli-2 from chimpanzee based on the immunizing sequence
Mouse reactivity reported in scientific literature (PMID: 27760825).

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GLI-2 Antibody

  • GLI family zinc finger 2
  • GLI2
  • GLI-2
  • GLI-Kruppel family member GLI2
  • glioma-associated oncogene family zinc finger 2
  • HPE9tax helper protein 1
  • oncogene GLI2
  • tax helper protein 2
  • Tax helper protein
  • tax-responsive element-2 holding protein
  • tax-responsive element-25-bp sequence binding protein
  • THP
  • THP1
  • THP2
  • zinc finger protein GLI2

Background

This protein belongs to the C2H2 type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes Greig cephalopolysyndactyly syndrome, Pallister Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLI-2 Antibody (NB600-874)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Chemotaxis, IHC, IHC-P, In Vivo, WB.


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Chemotaxis
(3)
IHC
(1)
IHC-P
(1)
In Vivo
(1)
WB
(1)
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Human
(3)
Mouse
(2)
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Showing Publications 1 - 9 of 9.
Publications using NB600-874 Applications Species
Shamsoon K, Hiraki D, Yoshida K et al. The Role of Hedgehog Signaling in the Melanoma Tumor Bone Microenvironment International Journal of Molecular Sciences 2023-05-16 [PMID: 37240209] (IHC) IHC
Juuri E, Tikka P, Domanskyi A et al. Ptch2 is a Potential Regulator of Mesenchymal Stem Cells Frontiers in Physiology 2022-04-28 [PMID: 35574464] (In Vivo) In Vivo
Xia L, Bouamar H, Gu X et al. Gli2 mediates the development of castration resistant prostate cancer Int. J. Oncol. 2020-04-13 [PMID: 32319599]
Mazzone A, Gibbons SJ, Eisenman ST et al. Direct repression of anoctamin 1 ( ANO1) gene transcription by Gli proteins FASEB J. 2019-02-25 [PMID: 30802137] (Chemotaxis, Human) Chemotaxis Human
Zhu H, Xia L, Shen Q et al. Differential effects of GLI2 and GLI3 in regulating cervical cancer malignancy in vitro and in vivo. Lab. Invest. 2018-07-02 [PMID: 29967343] (WB, Human) WB Human
Wu M, Ingram L, Tolosa EJ et al. Gli transcription factors mediate the oncogenic transformation of prostate basal cells induced by a Kras-androgen receptor axis. J. Biol. Chem. 2016-10-19 [PMID: 27760825] (Chemotaxis, Mouse) Chemotaxis Mouse
Jackson DA, Smith TD, Amarsaikhan N et al. Modulation of the IL-6 Receptor alpha Underlies GLI2-Mediated Regulation of Ig Secretion in Waldenstrom Macroglobulinemia Cells. J Immunol 2015-09-15 [PMID: 26238488]
Won Ky, Kim Gy, Lim Sj et al. Autophagy is related to the hedgehog signaling pathway in human gastric adenocarcinoma: Prognostic significance of Beclin-1 and Gli2 expression in human gastric adenocarcinoma Pathology - Research and Practice et al. 2014-11-20 [PMID: 25512258] (IHC-P, Human)

Details:
Gli-2 antibody used for IHC-P on human gastric adenocarcinoma tissue microarrays.
IHC-P Human
Feng R Hierarchical regulation of albumin transcription in liver cells under physiologic and pathophysiologic conditions Thesis 2022-01-01 (Chemotaxis, Mouse) Chemotaxis Mouse

Review for GLI-2 Antibody (NB600-874) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-874:
Filter by Applications
IHC-P
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GLI-2 NB600-874
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5
reviewed by:
Verified Customer
IHC-P Mouse 02/28/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedadenocarcinomas
SpeciesMouse
Lot33663

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Verified Customer
02/28/2021
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol GLI2
Entrez
Uniprot