GLI-2 Antibody

Images

 
Western Blot: GLI-2 Antibody [NB600-874] - Analysis of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains ...read more
Immunohistochemistry-Paraffin: GLI-2 Antibody [NB600-874] - Mouse adenocarcinoma section shows high and diffuse nuclear Gli2 expression. IHC-P image submitted by a verified customer review.
Flow (Intracellular): GLI-2 Antibody [NB600-874] - intracellular staining for GLI-2 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-2 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) ...read more
Immunohistochemistry: GLI-2 Antibody [NB600-874] - Staining of tumor cells in human breast tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ELISA, Flow-IC, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GLI-2 Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 46-60 of human GLI-2 (isoform a).
Localization
Nuclear
Specificity
NB600-874 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4. Tested by Western blotting on human lung, gives low background, but no band was detected.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLI2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA 1:10000-1:30000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Flow (Intracellular)
Application Notes
See figure legend for expectations by Western blot. Multiple splice variants have been reported for this protein a, b, g and d. Detection of Gli-2 by Western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely - be low. Furthermore, Gli-2 expression is likely (being developmentally regulated and induced, making it difficult) detected in whole tissue homogenates. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 27760825).
Reviewed Applications
Read 1 Review rated 5
using
NB600-874 in the following applications:

Publications
Read Publications using
NB600-874 in the following applications:

  • 2 publications
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Reacts with: Chimpanzee. Mouse reactivity reported in scientific literature (PMID: 27760825).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLI-2 Antibody

  • GLI family zinc finger 2
  • GLI2
  • GLI-2
  • GLI-Kruppel family member GLI2
  • glioma-associated oncogene family zinc finger 2
  • HPE9tax helper protein 1
  • oncogene GLI2
  • tax helper protein 2
  • Tax helper protein
  • tax-responsive element-2 holding protein
  • tax-responsive element-25-bp sequence binding protein
  • THP
  • THP1
  • THP2
  • zinc finger protein GLI2

Background

This protein belongs to the C2H2 type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes Greig cephalopolysyndactyly syndrome, Pallister Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GLI-2 Antibody (NB600-874)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ChIP, IHC-P, WB.


Filter By Application
ChIP
(2)
IHC-P
(1)
WB
(1)
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Human
(3)
Mouse
(1)
All Species

Review for GLI-2 Antibody (NB600-874) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-874:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GLI-2 NB600-874
Enlarge
5
reviewed by:
Anonymous
IHC-P Mouse 02/28/2021
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedadenocarcinomas
SpeciesMouse
Lot33663

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

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ChIP Video Protocol
ChIP Webinar

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
02/28/2021
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol GLI2
Entrez
Uniprot