GLI2 Products By Gene Name

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="GLI-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3635) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

5-001-A) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody (Catalog # [[catalogNumber:BAF016).Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # [[catalogNumber:MAG999) and DNA was purified using chelating resin solution. Thebcl-2promoter was detected by standard PCR." class="big_lightbox"> 5-001-A) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody (Catalog # [[catalogNumber:BAF016).Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # [[catalogNumber:MAG999) and DNA was purified using chelating resin solution. Thebcl-2promoter was detected by standard PCR." alt="Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-2/DNA complexes were immunoprecipitated using 5 µg Sheep Anti-Human GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3526) or control antibody (Catalog # 5-001-A) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody (Catalog # [[catalogNumber:BAF016).Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # [[catalogNumber:MAG999) and DNA was purified using chelating resin solution. Thebcl-2promoter was detected by standard PCR." class="big_thumb" />