Uromodulin Antibody

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Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175) followed by HRP-conjugated ...read more
Uromodulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175) at 10 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Uromodulin Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Uromodulin
Ser24-Ala618
Accession # Q91X17
Specificity
Detects mouse Uromodulin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human Uromodulin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
UMOD
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF5175 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Uromodulin Antibody

  • ADMCKD2
  • FJHN
  • HNFJ
  • HNFJ1
  • MCKD2
  • Tamm-Horsfall glycoprotein
  • Tamm-Horsfall urinary glycoprotein
  • THGP
  • THP
  • UMOD
  • uromodulin (uromucoid, Tamm-Horsfall glycoprotein)
  • Uromodulin
  • Uromucoid

Background

Uromodulin (also Tamm-Horsfall glycoprotein or THP) is a 105‑120 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑148), a ZP domain that mediates oligomerization (aa 335‑590), and a cleavable C-terminal propeptide (aa 619‑642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17 aa substitution for aa 601‑642, and a 166 aa substitution for aa 441‑642. Over aa 25‑618, mouse Uromodulin shares 78% and 89% aa identical to human and rat Uromodulin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Uromodulin Antibody (AF5175)(2)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: WB.


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WB
(2)
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(1)
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Bioinformatics

Gene Symbol UMOD
Entrez
Uniprot