Uromodulin Antibody

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Uromodulin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Uromodulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubule epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Uromodulin Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Uromodulin
  Ser24-Ala618
  Accession # Q91X17
• Specificity: Detects mouse Uromodulin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human Uromodulin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: UMOD
• Purity: Immunogen affinity purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Uromodulin Antibody

• ADMCKD2
• FJHN
• HNFJ
• HNFJ1
• MCKD2
• Tamm-Horsfall glycoprotein
• Tamm-Horsfall urinary glycoprotein
• THGP
• THP
• UMOD
• uromodulin (uromucoid, Tamm-Horsfall glycoprotein)
• Uromodulin
• Uromucoid

Background

Uromodulin (also Tamm-Horsfall glycoprotein or THP) is a 105‑120 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑148), a ZP domain that mediates oligomerization (aa 335‑590), and a cleavable C-terminal propeptide (aa 619‑642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17 aa substitution for aa 601‑642, and a 166 aa substitution for aa 441‑642. Over aa 25‑618, mouse Uromodulin shares 78% and 89% aa identical to human and rat Uromodulin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Uromodulin Antibody (AF5175)(2)

Publications using AF5175

• O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy Physiol Rep, 2018;6(6):e13654. 2018 [PMID: 29595914] (WB,, Mouse)
• S Mary, HY Small, J Siwy, W Mullen, A Giri, C Delles Polymerization-Incompetent Uromodulin in the Pregnant Stroke-Prone Spontaneously Hypertensive Rat Hypertension, 2017;69(5):910-918. 2017 [PMID: 28348009] (WB,, Rat)

Bioinformatics

• Gene Symbol: UMOD
• Entrez:
  • 7369(Human)
  • 22242(Mouse)
  • 25128(Rat)
• Uniprot:
  • Q91X17()