GLI-2 Antibodies
We offer GLI-2 Antibodies for use in common research applications: Chromatin Immunoprecipitation (ChIP), ELISA, Flow (Intracellular), Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each GLI-2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GLI-2 Antibodies can be used in a variety of model species: Human, Mouse, Primate, Rat. Use the list below to choose the GLI-2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GLI-2 polyclonal antibodies and browse our GLI-2 monoclonal antibody catalog.
Alternate Names for GLI-2 Antibodies
anti-GLI-2 antibody, anti-GLI2 antibody, anti-GLI family zinc finger 2 antibody, anti-GLI-Kruppel family member GLI2 antibody, anti-glioma-associated oncogene family zinc finger 2 antibody, anti-HPE9tax helper protein 1 antibody, anti-oncogene GLI2 antibody, anti-tax helper protein 2 antibody, anti-Tax helper protein antibody, anti-tax-responsive element-2 holding protein antibody, anti-tax-responsive element-25-bp sequence binding protein antibody, anti-THP antibody, anti-THP1 antibody, anti-THP2 antibody, anti-zinc finger protein GLI2 antibody
16 Results for "gli-2" in Primary Antibodies
5-001-A) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody (Catalog # [[catalogNumber:BAF016).Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # [[catalogNumber:MAG999) and DNA was purified using chelating resin solution. Thebcl-2promoter was detected by standard PCR." alt="Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-2/DNA complexes were immunoprecipitated using 5 µg Sheep Anti-Human GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3526) or control antibody (Catalog # 5-001-A) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody (Catalog # [[catalogNumber:BAF016).Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # [[catalogNumber:MAG999) and DNA was purified using chelating resin solution. Thebcl-2promoter was detected by standard PCR." class="big_thumb" />
VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="GLI-2 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human GLI-2 Monoclonal Antibody (Catalog # MAB35261) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
![The PY-type nuclear localization signal (PY-NLS) is required for Gli activation.(A) Western blot of cell lysates from the indicated cell lines indicated that Myc-Gli2WT and Myc-Gli2mPY were expressed at comparable levels that were slightly higher than that of endogenous Gli2. (B–C) Normalized mRNA levels of endogenous Gli1 (B) or Patch1 (C) measured by quantitative reverse transcription PCR (RT-qPCR) in control (green fluorescent protein [GFP] short hairpin RNA [shRNA]) or Gli2-depeleted NIH3T3 cells with or without lentiviral infection of the indicated Gli2 constructs. (D) Fractionation of Myc-Gli2WT and Myc-Gli2mPY from the indicated cell lines treated with or without SAG. Quantification of protein level is shown in the bottom panel. (E–F) Immunostaining (E) and quantification (F) of ciliary-localized Myc-Gli2WT or Myc-Gli2mPY in NIH3T3mGli2-shRNA cells treated with or without sonic hedgehog (Shh). N = 50 cells were examined for each Gli construct. Data are means ± SD from 2 independent experiments. **P < 0.01. (G) Gli-luciferase assay was performed in NIH3T3 cells transfected with the indicated constructs. Data are means ± SD from 2 independent experiments. *P < 0.05, NS: not significant. The underlying data for this figure can be found in S1 Data. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28777795), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3635_mouse-gli-2-affinity-purified-polyclonal-ab-western-blot-12122025749213.jpg)
![The PY-type nuclear localization signal (PY-NLS) is required for Gli activation.(A) Western blot of cell lysates from the indicated cell lines indicated that Myc-Gli2WT and Myc-Gli2mPY were expressed at comparable levels that were slightly higher than that of endogenous Gli2. (B–C) Normalized mRNA levels of endogenous Gli1 (B) or Patch1 (C) measured by quantitative reverse transcription PCR (RT-qPCR) in control (green fluorescent protein [GFP] short hairpin RNA [shRNA]) or Gli2-depeleted NIH3T3 cells with or without lentiviral infection of the indicated Gli2 constructs. (D) Fractionation of Myc-Gli2WT and Myc-Gli2mPY from the indicated cell lines treated with or without SAG. Quantification of protein level is shown in the bottom panel. (E–F) Immunostaining (E) and quantification (F) of ciliary-localized Myc-Gli2WT or Myc-Gli2mPY in NIH3T3mGli2-shRNA cells treated with or without sonic hedgehog (Shh). N = 50 cells were examined for each Gli construct. Data are means ± SD from 2 independent experiments. **P < 0.01. (G) Gli-luciferase assay was performed in NIH3T3 cells transfected with the indicated constructs. Data are means ± SD from 2 independent experiments. *P < 0.05, NS: not significant. The underlying data for this figure can be found in S1 Data. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28777795), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3635_mouse-gli-2-affinity-purified-polyclonal-ab-western-blot-121220257455211.jpg)
![Western Blot: GLI-2 Antibody [NB600-874] - Analysis of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature (800 nm channel, green).](http://images.novusbio.com/fullsize/GLI-2-Antibody-Western-Blot-NB600-874-img0012.jpg "Western Blot: GLI-2 Antibody [NB600-874] - Analysis of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature (800 nm channel, green).")
![Immunohistochemistry-Paraffin: GLI-2 Antibody [NB600-874] - Mouse adenocarcinoma section shows high and diffuse nuclear Gli2 expression. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-Paraffin-NB600-874-img0013.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): GLI-2 Antibody [NB600-874] - intracellular staining for GLI-2 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-2 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Flow-Intracellular-NB600-874-img0006.jpg)
![Immunohistochemistry: GLI-2 Antibody [NB600-874] - Staining of tumor cells in human breast tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-NB600-874-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0007.jpg "Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: GLI-2 Antibody [NBP2-23602] - GLI-2 (red) and DAPI (blue) in mouse BT-474 primary tumors (breast cancer tissue). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-Paraffin-NBP2-23602-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - GLI-2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:50 was used. Image objective 40x.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0004.jpg)
![Immunohistochemistry: GLI-2 Antibody [NBP2-23602] - Analysis of GLI-2 in mouse heart.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-NBP2-23602-img0005.jpg)
![Immunohistochemistry-Paraffin: GLI-2 Antibody (524409) [Unconjugated] [MAB35262] - GLI-2 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human GLI-2 Monoclonal Antibody (Catalog # MAB35262) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/GLI-2-Antibody-524409-Unconjugated-Immunohistochemistry-Paraffin-MAB35262-img0001.jpg "Immunohistochemistry-Paraffin: GLI-2 Antibody (524409) [Unconjugated] [MAB35262] - GLI-2 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human GLI-2 Monoclonal Antibody (Catalog # MAB35262) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.")
![Western Blot: GLI-2 Antibody [NBP3-05613] - Western blot analysis of extracts of various cell lines, using GLI-2 antibody (NBP3-05613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Western-Blot-NBP3-05613-img0001.jpg "Western Blot: GLI-2 Antibody [NBP3-05613] - Western blot analysis of extracts of various cell lines, using GLI-2 antibody (NBP3-05613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation:GLI-2 AntibodyNBP3-29457] -Detection of human GLI2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLI2 antibody NBP3-29457 (lot NBP3-29457-1) used for IP at 6 µg per reaction. GLI2 was also immunoprecipitated by rabbit anti-GLI2 antibodies. For blotting immunoprecipitated GLI2, NBP3-29457 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29457_rabbit-gli-2-pab-301220241652440.jpg "Immunoprecipitation:GLI-2 AntibodyNBP3-29457] -Detection of human GLI2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLI2 antibody NBP3-29457 (lot NBP3-29457-1) used for IP at 6 µg per reaction. GLI2 was also immunoprecipitated by rabbit anti-GLI2 antibodies. For blotting immunoprecipitated GLI2, NBP3-29457 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
