Girdin Antibody [Unconjugated]

Western blot shows lysates of H69AR human small cell lung cancer cell line, MRC-5 human embryonic lung fibroblast cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Girdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5345) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Girdin at approximately 200 to 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Girdin at approximately 206 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Girdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5345) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Girdin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Girdin
  Asn1510-Leu1824
  Accession # Q3V6T2
• Specificity: Detects endogenous human Girdin in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CCDC88A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Girdin Antibody [Unconjugated]

• Akt phosphorylation enhancer
• APE
• APEGIRDIN
• CCDC88A
• coiled-coil domain containing 88A
• Coiled-coil domain-containing protein 88A
• G alpha-interacting vesicle-associated protein
• G{alpha}-interacting vesicle-associated protein
• Girders of actin filament
• Girdin
• GIV
• GIVFLJ10392
• GRDN
• GRDNAKT-phosphorylation enhancer
• HkRP1
• HkRP1Galpha-interacting vesicle-associated protein
• Hook-related protein 1
• KIAA1212DKFZp686D0630

Background

Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Girdin Antibody (AF5345)(8)

Publications using AF5345

• Fuyang Cao, Desong Yang, Feiyu Tang, Can Lu, Xiang He, Songming Chen et al. Girdin Promotes Tumorigenesis and Chemoresistance in Lung Adenocarcinoma by Interacting with PKM2 Cancers (Basel) 2022-11-19 [PMID: 36428781]
• Xiaoze Wang, Atsushi Enomoto, Liang Weng, Yasuyuki Mizutani, Shaniya Abudureyimu, Nobutoshi Esaki et al. Girdin/GIV regulates collective cancer cell migration by controlling cell adhesion and cytoskeletal organization Cancer Science 2018-11-01 [PMID: 30194792] (Western Blot, Immunocytochemistry, Human)
• Liang Weng, Yi-Peng Han, Atsushi Enomoto, Yasuyuki Kitaura, Shushi Nagamori, Yoshikatsu Kanai et al. Negative regulation of amino acid signaling by MAPK-regulated 4F2hc/Girdin complex PLOS Biology 2018-03-14 [PMID: 29538402]
• Yamamura Y, Asai N, Enomoto A, Kato T, Mii S, Kondo Y, Ushida K, Niimi K, Tsunoda N, Nagino M, Ichihara S, Furukawa K, Maeda K, Murohara T, Takahashi M Akt-Girdin signaling in cancer-associated fibroblasts contributes to tumor progression. Cancer Res, 2015-03-01;75(5):813-23. 2015-03-01 [PMID: 25732845] (IHC-P, Human)
• Ota , Haruko, Hikita , Takao, Sawada , Masato, Nishioka , Tomoki, Matsumoto , Mami, Komura , Masayuki, Ohno , Akihisa, Kamiya , Yukiyo, Miyamoto , Takuya, Asai , Naoya, Enomoto , Atsushi, Takahashi , Masahide, Kaibuchi , Kozo, Sobue , Kazuya, Sawamoto , Kazunobu Speed control for neuronal migration in the postnatal brain by Gmip-mediated local inactivation of RhoA. Nat Commun, 2014-07-30;5(0):4532. 2014-07-30 [PMID: 25074242] (Western Blot, Mouse)
• Tomiyama , L, Sezaki , T, Matsuo , M, Ueda , K, Kioka , N Loss of Dlg5 expression promotes the migration and invasion of prostate cancer cells via Girdin phosphorylation. Oncogene, 2014-03-24;34(9):1141-9. 2014-03-24 [PMID: 24662825] (Western Blot, Human)
• Ota H, Hikita T, Nishioka T, Matsumoto M, Ito J, Asai N, Enomoto A, Takahashi M, Kaibuchi K, Sobue K, Sawamoto K Proteomic analysis of Girdin-interacting proteins in migrating new neurons in the postnatal mouse brain. Biochem Biophys Res Commun, 2013-11-06;442(1):16-21. 2013-11-06 [PMID: 24211587] (IHC, Mouse)
• Ito T, Komeima K, Yasuma T, Enomoto A, Asai N, Asai M, Iwase S, Takahashi M, Terasaki H Girdin and its phosphorylation dynamically regulate neonatal vascular development and pathological neovascularization in the retina. Am J Pathol, 2012-11-27;182(2):586-96. 2012-11-27 [PMID: 23195430] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CCDC88A
• Uniprot:
  • Q3V6T2()