Girdin Antibody [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

Girdin Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Girdin
  Asn1510-Leu1824
  Accession # Q3V6T2
• Specificity: Detects endogenous human Girdin in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Girdin Antibody [Alexa Fluor® 594]

• Akt phosphorylation enhancer
• APE
• APEGIRDIN
• CCDC88A
• coiled-coil domain containing 88A
• Coiled-coil domain-containing protein 88A
• G alpha-interacting vesicle-associated protein
• G{alpha}-interacting vesicle-associated protein
• Girders of actin filament
• Girdin
• GIV
• GIVFLJ10392
• GRDN
• GRDNAKT-phosphorylation enhancer
• HkRP1
• HkRP1Galpha-interacting vesicle-associated protein
• Hook-related protein 1
• KIAA1212DKFZp686D0630

Background

Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.