Girdin Antibody [Biotin]

Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Girdin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5345) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Girdin at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Girdin Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Girdin
  Asn1510-Leu1824
  Accession # Q3V6T2
• Specificity: Detects human Girdin in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CCDC88A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Girdin Antibody [Biotin]

• Akt phosphorylation enhancer
• APE
• APEGIRDIN
• CCDC88A
• coiled-coil domain containing 88A
• Coiled-coil domain-containing protein 88A
• G alpha-interacting vesicle-associated protein
• G{alpha}-interacting vesicle-associated protein
• Girders of actin filament
• Girdin
• GIV
• GIVFLJ10392
• GRDN
• GRDNAKT-phosphorylation enhancer
• HkRP1
• HkRP1Galpha-interacting vesicle-associated protein
• Hook-related protein 1
• KIAA1212DKFZp686D0630

Background

Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196‑425 and 458‑1358), and one phosphoinositide-binding domain (aa 1390‑1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCDC88A
• Uniprot:
  • Q3V6T2()