G protein alpha inhibitor 1 Antibody (2B8-2A5)

Images

 
Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - GNAI1 monoclonal antibody (M01), clone 2B8-2A5. Analysis of GNAI1 expression in human liver.
Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Analysis of monoclonal antibody to GNAI1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Analysis of GNAI1 expression in transfected 293T cell line by GNAI1 monoclonal antibody (M01), clone 2B8-2A5.Lane 1: GNAI1 transfected ...read more
Sandwich ELISA: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Detection limit for recombinant GST tagged GNAI1 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, S-ELISA
Clone
2B8-2A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

G protein alpha inhibitor 1 Antibody (2B8-2A5) Summary

Immunogen
GNAI1 (AAH26326, 1 a.a. ~ 354 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVAAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYQEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
Specificity
GNAI1 - guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GNAI1
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.
Control
G protein alpha inhibitor 1 Knockout HeLa Cell Lysate
Publications
Read Publication using H00002770-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for G protein alpha inhibitor 1 Antibody (2B8-2A5)

  • Adenylate cyclase-inhibiting G alpha protein
  • Gi
  • Gi1 protein alpha subunit
  • guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 1
  • guanine nucleotide-binding protein G(i) subunit alpha-1

Background

Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for G protein alpha inhibitor 1 Antibody (H00002770-M01)(1)


Showing Publication 1 - 1 of 1.
Publication using H00002770-M01 Applications Species
Alain M, Marie-yvonne AN., et al. GI protein phosphorylation as marker for scoliosis progression, methods of increasing gipcr signaling subjects. United States Patent Application May 19 2016

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol GNAI1
Entrez
OMIM
Uniprot