Girdin Antibody

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Western blot shows lysates of H69AR human small cell lung cancer cell line, MRC-5 human embryonic lung fibroblast cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Girdin Antibody Summary

Immunogen
E. coli-derived recombinant human Girdin
Asn1510-Leu1824
Accession # Q3V6T2
Specificity
Detects endogenous human Girdin in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CCDC88A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5345 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Girdin Antibody

  • Akt phosphorylation enhancer
  • APE
  • APEGIRDIN
  • CCDC88A
  • coiled-coil domain containing 88A
  • Coiled-coil domain-containing protein 88A
  • G alpha-interacting vesicle-associated protein
  • G{alpha}-interacting vesicle-associated protein
  • Girders of actin filament
  • Girdin
  • GIV
  • GIVFLJ10392
  • GRDN
  • GRDNAKT-phosphorylation enhancer
  • HkRP1
  • HkRP1Galpha-interacting vesicle-associated protein
  • Hook-related protein 1
  • KIAA1212DKFZp686D0630

Background

Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196 - 425 and 458 - 1358), and one phosphoinositide-binding domain (aa 1390 - 1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF5345
Species: Hu
Applications: WB

Publications for Girdin Antibody (AF5345)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC Paraffin-embedded, WB.


Filter By Application
IHC
(1)
IHC Paraffin-embedded
(1)
WB
(3)
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Human
(3)
Mouse
(2)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF5345 Applications Species
Yamamura Y, Asai N, Enomoto A, Kato T, Mii S, Kondo Y, Ushida K, Niimi K, Tsunoda N, Nagino M, Ichihara S, Furukawa K, Maeda K, Murohara T, Takahashi M Akt-Girdin signaling in cancer-associated fibroblasts contributes to tumor progression. Cancer Res, 2015;75(5):813-23. 2015 [PMID: 25732845] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Ota, Haruko, Hikita, Takao, Sawada, Masato, Nishioka, Tomoki, Matsumoto, Mami, Komura, Masayuki, Ohno, Akihisa, Kamiya, Yukiyo, Miyamoto, Takuya, Asai, Naoya, Enomoto, Atsushi, Takahashi, Masahide, Kaibuchi, Kozo, Sobue, Kazuya, Sawamoto, Kazunobu Speed control for neuronal migration in the postnatal brain by Gmip-mediated local inactivation of RhoA. Nat Commun, 2014;5(0):4532. 2014 [PMID: 25074242] (WB,, Mouse) WB Mouse
Tomiyama, L, Sezaki, T, Matsuo, M, Ueda, K, Kioka, N Loss of Dlg5 expression promotes the migration and invasion of prostate cancer cells via Girdin phosphorylation. Oncogene, 2015;34(9):1141-9. 2015 [PMID: 24662825] (WB,, Human) WB Human
Ota H, Hikita T, Nishioka T, Matsumoto M, Ito J, Asai N, Enomoto A, Takahashi M, Kaibuchi K, Sobue K, Sawamoto K Proteomic analysis of Girdin-interacting proteins in migrating new neurons in the postnatal mouse brain. Biochem Biophys Res Commun, 2013;442(1):16-21. 2013 [PMID: 24211587] (IHC,, Mouse) IHC Mouse
Ito T, Komeima K, Yasuma T, Enomoto A, Asai N, Asai M, Iwase S, Takahashi M, Terasaki H Girdin and its phosphorylation dynamically regulate neonatal vascular development and pathological neovascularization in the retina. Am J Pathol, 2013;182(2):586-96. 2013 [PMID: 23195430] (WB,, Human) WB Human

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Bioinformatics

Gene Symbol CCDC88A
Entrez
Uniprot