Gemin 3 Antibody

Immunohistochemistry:[NBP3-29300]-Detection of human DDX20 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX20.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Gemin 3 Antibody Summary

• Description: Novus Biologicals Rabbit Gemin 3 Antibody (NBP3-29300) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 600 and 650
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX20
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-buffered Saline 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Gemin 3 Antibody

• Component of gems 3
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
• DEAD box protein 20
• DEAD box protein DP 103
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD
• DKFZp434H052
• DP103DEAD-box protein DP103
• EC 3.6.4.13
• Gemin-3
• GEMIN3gemin-3
• probable ATP-dependent RNA helicase DDX20
• SMN-interacting protein

Background

The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly. In the nucleus, SMN is required for pre-mRNA splicing, likely by serving in the regeneration of snRNPs. A DEAD box putative RNA helicase, named Gemin 3 which is another component of the SMN complex, has been identified. Gemin 3 interacts directly with SMN, as well as with SmB, SmD2 and SmD3. Immunolocalization studies using mAbs to Gemin 3 show that it colocalizes with SMN in gems. Gemin 3 binds SMN via its unique COOH-terminal domain, and SMN mutations found in some SMA patients strongly reduce this interaction. The presence of a DEAD box motif in Gemin 3 suggests that it may provide the catalytic activity that plays a critical role in the function of the SMN complex on RNPs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX20