Gemin 3 Antibody - BSA Free

Western Blot: Gemin 3 Antibody [NBP2-16638] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:500.

Immunohistochemistry-Paraffin: Gemin 3 Antibody [NBP2-16638] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Gemin 3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Gemin 3 Antibody - BSA Free (NBP2-16638) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Gemin 3. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus; gem
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX20
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Gemin 3 Antibody - BSA Free

• Component of gems 3
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
• DEAD box protein 20
• DEAD box protein DP 103
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD
• DKFZp434H052
• DP103DEAD-box protein DP103
• EC 3.6.4.13
• Gemin-3
• GEMIN3gemin-3
• probable ATP-dependent RNA helicase DDX20
• SMN-interacting protein

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DDX20
• Uniprot:
  • Q9UHI6(Human)