Gemin 3 Antibody

Images

 
Western Blot: Gemin 3 Antibody [NBP2-16638] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: Gemin 3 Antibody [NBP2-16638] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.65 mg/ml

Order Details

Gemin 3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Gemin 3. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus; gem
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX20
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.65 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Gemin 3 Antibody

  • Component of gems 3
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
  • DEAD box protein 20
  • DEAD box protein DP 103
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD
  • DKFZp434H052
  • DP103DEAD-box protein DP103
  • EC 3.6.4.13
  • Gemin-3
  • GEMIN3gemin-3
  • probable ATP-dependent RNA helicase DDX20
  • SMN-interacting protein

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DDX20
Uniprot