Gemin 3 Antibody

Images

 
Western Blot: Gemin 3 Antibody [NB100-1801] - Detection of Human DDX20 on HeLa whole cell lystate using NB100-1801. DDX20 was also immunoprecipitated using rabbit anti-DDX20 antibody NB100-1802 and two.
Immunohistochemistry: Gemin 3 Antibody [NB100-1801] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX20 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

       

Order Details

Gemin 3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human DEAD (Asp-Glu-Ala-Asp) Box 20 using the numbering given in entry NP_009135.3 (GeneID 11218).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX20
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-4 ug/mg of lysate
Positive Control
Gemin 3 Lysate (NBL1-09786)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Gemin 3 Antibody

  • Component of gems 3
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
  • DEAD box protein 20
  • DEAD box protein DP 103
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD
  • DKFZp434H052
  • DP103DEAD-box protein DP103
  • EC 3.6.4.13
  • Gemin-3
  • GEMIN3gemin-3
  • probable ATP-dependent RNA helicase DDX20
  • SMN-interacting protein

Background

DDX20 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAD box proteins also exhibit functions involved in transcriptional regulation. In addition to its role in snRNP assembly, DDX20 can function as a transcriptional repressor. DDX20 is also known as probable ATP-dependent RNA helicase DDX20, DEAD box protein 20, DEAD box protein DP 103, component of gems 3, gemin-3, DP103, and DKFZp434H052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DDX20
Entrez
Uniprot