Gemin 3 Antibody (5H5)

Immunocytochemistry/Immunofluorescence: Gemin 3 Antibody (5H5) [H00011218-M01] - Analysis of monoclonal antibody to DDX20 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: Gemin 3 Antibody (5H5) [H00011218-M01] - Detection limit for recombinant GST tagged DDX20 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: ELISA, ICC/IF
• Clone: 5H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Gemin 3 Antibody (5H5) Summary

• Immunogen: DDX20 (NP_009135, 725 a.a. ~ 824 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGASQRAKQSRRNLPRRSSFRLQTEAQEDDWYDCHREIRLSFSDTYQDYEEYWRAYYRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTIYLQEMMHSNQ
• Specificity: DDX20 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDX20
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Sandwich ELISA
• Application Notes: Antibody reactive against recombinant protein on ELISA. GST alone used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Gemin 3 Antibody (5H5)

• Component of gems 3
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
• DEAD box protein 20
• DEAD box protein DP 103
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD
• DKFZp434H052
• DP103DEAD-box protein DP103
• EC 3.6.4.13
• Gemin-3
• GEMIN3gemin-3
• probable ATP-dependent RNA helicase DDX20
• SMN-interacting protein

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX20
• Entrez:
  • 11218(Human)
• OMIM:
  • 606168(Human)
• Uniprot:
  • NP_009135(Human)