GDF-8/Myostatin Overexpression Lysate

Images

 
Western Blot: GDF8/Myostatin Overexpression Lysate (Adult Normal) [NBL1-13340] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GDF8/Myostatin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GDF-8/Myostatin Overexpression Lysate Summary

Description

GDF-8/Myostatin Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210368

Accession#: NM_005259

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
MSTN

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
42.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBL1-13340.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for GDF-8/Myostatin Overexpression Lysate

  • GDF8
  • GDF-8
  • GDF8growth differentiation factor 8
  • growth/differentiation factor 8
  • MSLHP
  • MSTN
  • Myostatin

Background

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

291-G1
Species: Hu
Applications: BA
DFN00
Species: Hu
Applications: ELISA
NB100-56511
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, WB
NBP2-76836
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
MAB66861
Species: Hu
Applications: ICC, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
1958-GD
Species: Hu, Mu, Rt
Applications: BA
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP1-89953
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
AF339
Species: Hu
Applications: Block, IHC, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
M6000B
Species: Mu
Applications: ELISA
292-G2
Species: Hu
Applications: BA
AF3797
Species: Hu, Mu
Applications: ChIP, ICC, IHC, Simple Western, WB
NB600-607
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
AF5366
Species: Hu, Mu, Rt
Applications: Simple Western, WB
NBP2-44245
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
355-BM
Species: Hu, Mu, Rt
Applications: BA
NBL1-13340
Species: Hu
Applications: WB

Publications for GDF-8/Myostatin Lysate (NBL1-13340)(1)

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
All Species

Reviews for GDF-8/Myostatin Lysate (NBL1-13340) (0)

There are no reviews for GDF-8/Myostatin Lysate (NBL1-13340). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GDF-8/Myostatin Lysate (NBL1-13340) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GDF-8/Myostatin Products

Research Areas for GDF-8/Myostatin Lysate (NBL1-13340)

Find related products by research area.

Blogs on GDF-8/Myostatin

There are no specific blogs for GDF-8/Myostatin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GDF-8/Myostatin Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol MSTN