788-G8), Recombinant Human/Mouse/Rat GDF-11/BMP-11 (
1958-GD), Recombinant Human BMP-6 (
507-BP), and Recombinant Mouse BMP-6 (
6325-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (
HAF109). A specific band was detected for GDF-8/Myostatin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_lightbox">

788-G8), Recombinant Human/Mouse/Rat GDF-11/BMP-11 (
1958-GD), Recombinant Human BMP-6 (
507-BP), and Recombinant Mouse BMP-6 (
6325-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (
HAF109). A specific band was detected for GDF-8/Myostatin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human/Mouse/Rat GDF-8/Myostatin (
788-G8), Recombinant Human/Mouse/Rat GDF-11/BMP-11 (
1958-GD), Recombinant Human BMP-6 (
507-BP), and Recombinant Mouse BMP-6 (
6325-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (
HAF109). A specific band was detected for GDF-8/Myostatin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_thumb" />
788-G8) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Mouse GDF-8/Myostatin (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788). The ND
50 is typically 0.6-3 µg/mL." class="big_lightbox">

788-G8) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Mouse GDF-8/Myostatin (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788). The ND
50 is typically 0.6-3 µg/mL." class="big_thumb" />