GDF-8/Myostatin Antibody (84231) - Propeptide

• Reactivity: Mu
• Applications: WB
• Clone: 84231
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

GDF-8/Myostatin Antibody (84231) - Propeptide Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse GDF-8
  Asn25-Ser376
  Accession # O08689
• Specificity: Detects mouse GDF-8 propeptide in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) GDF‑1 propeptide, rmGDF-3 propeptide, rmGDF-5, or rmGDF-6 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: MSTN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-8/Myostatin Antibody (84231) - Propeptide

• GDF8
• GDF-8
• GDF8growth differentiation factor 8
• growth/differentiation factor 8
• MSLHP
• MSTN
• Myostatin

Background

Growth Differentiation Factor 8 (GDF-8), also known as Myostatin, is a secreted TGF-beta superfamily protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass (1‑3). Mouse GDF-8 is synthesized as a 376 amino acid (aa) preproprotein that consists of a 24 aa signal peptide, a 243 aa propeptide, and a 109 aa mature protein (2). Within the propeptide, mouse GDF-8 shares 96% and 99% aa sequence identity with human and rat GDF-8, respectively. GDF-8 is secreted as a preproprotein that is cleaved by BMP-1 family proteases to separate the 35‑40 kDa propeptide from the 12 kDa bioactive mature protein (4‑6). This results in a latent complex containing a disulfide-linked dimer of the mature protein and two noncovalently-associated molecules of the propeptide (2, 6). The GDF-8 propeptide functions as an inhibitor of mature GDF-8, and GDF-8 activity can also be inhibited through association with Follistatin, FLRG, Decorin, or GASP-1 (6‑11). The uncleaved GDF-8 proprotein binds Latent TGF-beta bp3 which can sequester it in the extracellular matrix and prevent the proteolytic cleavage of the propeptide (12). GDF-8 binds to the type II Activin receptor Activin RIIB which then associates with the type I receptors Activin RIB/ALK-4 or TGF-beta RI/ALK-5 to induce signaling (13). GDF-8 additionally inhibits adipogenic differentiation of mesenchymal stem cells and preadipocytes (14). Genetic deletion of GDF-8 or in vivo administration of the GDF-8 propeptide induces muscle hypertrophy as well as enhanced glucose utilization and insulin sensitivity and a reduction in overall fat mass (15, 16).

1. McPherron, A.C. (2010) Immunol. Endocr. Metab. Agents Med. Chem. 10:217.
2. McPherron, A.C. et al. (1997) Nature 387:83.
3. Zimmers, T.A. et al. (2002) Science 296:1486.
4. Wolfman, N.M. et al. (2003) Proc. Natl. Acad. Sci. 100:15842.
5. McFarlane, C. et al. (2005) Dev. Biol. 283:58.
6. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. 98:9306.
7. Thies, R.S. et al. (2001) Growth Factors 18:251.
8. Amthor, H. et al. (2004) Dev. Biol. 270:19.
9. Hill, J.J. et al. (2002) J. Biol. Chem. 277:40735.
10. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
11. Hill, J.J. et al. (2003) Molecular Endocrinology 17:1144.
12. Anderson, S.B. et al. (2008) J. Biol. Chem. 283:7027.
13. Rebbapragada, A. et al. (2003) Mol. Cell. Biol. 23:7230.
14. Guo, W. et al. (2008) J. Biol. Chem. 283:9136.
15. Matsakas, A. et al. (2009) Neuromuscul. Disord. 19:489.
16. Guo, T. et al. (2009) PloS ONE 4:e4937.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GDF-8/Myostatin Antibody (MAB7881)(2)

Publications using MAB7881

• Estibaliz Castillero, Ziad A. Ali, Hirokazu Akashi, Nicholas Giangreco, Catherine Wang, Eric J. Stöhr et al. Structural and functional cardiac profile after prolonged duration of mechanical unloading: potential implications for myocardial recovery American Journal of Physiology-Heart and Circulatory Physiology 11/1/2018 [PMID: 30141986]
• Tamas Kocsis Myostatin propeptide mutation of the hypermuscular Compact mice decreases the formation of myostatin and improves insulin sensitivity Am. J. Physiol. Endocrinol. Metab, 2016-12-13;0(0):ajpendo.00216. 2016-12-13 [PMID: 27965203] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: MSTN
• Uniprot:
  • O08689()