GDF-11/GDF-8 Antibody (84214) [Unconjugated]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 84214
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

GDF-11/GDF-8 Antibody (84214) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse GDF-8
  Asn25-Ser376
  Accession # O08689
• Specificity: Detects human, mouse, and rat GDF-8 in direct ELISAs and Western blots. In Western blots, this antibody shows approximately 30% cross-reactivity with recombinant human/mouse/rat GDF-11. In direct ELISAs, this antibody shows less than 1% cross-reactivity with recombinant mouse (rm) GDF-1, rmGDF-3, rmGDF-5, rmGDF-6, and rmGDF-9.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: GDF11
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-11/GDF-8 Antibody (84214) [Unconjugated]

• BMP11
• BMP-11
• bone morphogenetic protein 11
• GDF-11
• GDF-11/GDF-8
• growth/differentiation factor 11
• VHO

Background

GDF-8, also known as Myostatin, belongs to the TGF-beta superfamily. It is synthesized as an inactive proprotein with the N-terminal pro-region and the C-terminal mature bioactive region. The GDF-8 proregion is capable of associating with active GDF-8 with high affinity and is a potent GDF-8 antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GDF-11/GDF-8 Antibody (MAB788)(12)

Publications using MAB788

• Sadhana A. Samant, Vinodkumar B. Pillai, Mahesh P. Gupta Skeletal muscle‐specific over‐expression of the nuclear sirtuin SIRT6 blocks cancer‐associated cachexia by regulating multiple targets JCSM Rapid Communications 1/1/2021 [PMID: 34212132]
• J Liu, M Pan, D Huang, Y Guo, M Yang, W Zhang, K Mai Myostatin-1 Inhibits Cell Proliferation by Inhibiting the mTOR Signal Pathway and MRFs, and Activating the Ubiquitin-Proteasomal System in Skeletal Muscle Cells of Japanese Flounder Paralichthys olivaceus Cells, 2020-10-29;9(11):. 2020-10-29 [PMID: 33138208] (Western Blot, Paralichthys olivaceus (Japanese Flounder))
• C Pan, N Jaiswal Ag, Y Zulia, S Singh, K Sha, JL Mohler, KH Eng, J Chakkalaka, JJ Krolewski, KL Nastiuk Prostate tumor-derived GDF11 accelerates androgen deprivation therapy-induced sarcopenia JCI Insight, 2020-03-26;0(0):. 2020-03-26 [PMID: 32078585] (Western Blot, Mouse)
• SA Samant, A Kanwal, VB Pillai, R Bao, MP Gupta The histone deacetylase SIRT6 blocks myostatin expression and development of muscle atrophy Sci Rep, 2017-09-19;7(1):11877. 9/19/2017 [PMID: 28928419]
• O Miroshnych, WT Chang, JL Dragoo The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration Am J Sports Med, 2016-12-27;45(4):945-953. 2016-12-27 [PMID: 28027451] (Western Blot, Human)
• Sang Beum Lee, Jeong Hwan Kim, Deuk-Hee Jin, Hyung-Joo Jin, Yong Soo Kim Myostatin inhibitory region of fish (Paralichthys olivaceus) myostatin-1 propeptide Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 4/1/2016 [PMID: 26827850]
• Lawlor M, Viola M, Meng H, Edelstein R, Liu F, Yan K, Luna E, Lerch-Gaggl A, Hoffmann R, Pierson C, Buj-Bello A, Lachey J, Pearsall S, Yang L, Hillard C, Beggs A Differential muscle hypertrophy is associated with satellite cell numbers and Akt pathway activation following activin type IIB receptor inhibition in Mtm1 p.R69C mice. Am J Pathol, 2014-04-13;184(6):1831-42. 2014-04-13 [PMID: 24726641] (Western Blot, Mouse)
• Lawlor MW, Read BP, Edelstein R, Yang N, Pierson CR, Stein MJ, Wermer-Colan A, Buj-Bello A, Lachey JL, Seehra JS, Beggs AH Inhibition of activin receptor type IIB increases strength and lifespan in myotubularin-deficient mice. Am. J. Pathol., 2011-02-01;178(2):784-93. 2011-02-01 [PMID: 21281811] (Western Blot, Mouse)
• Yamada M, Tatsumi R, Yamanouchi K, Hosoyama T, Shiratsuchi S, Sato A, Mizunoya W, Ikeuchi Y, Furuse M, Allen RE High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo. Am. J. Physiol., Cell Physiol., 2009-12-09;298(3):C465-76. 2009-12-09 [PMID: 20007454] (Western Blot, Rat)
• Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7. J. Mol. Biol., 2008-07-02;381(4):1025-39. 2008-07-02 [PMID: 18621057] (ELISA Development, Human)
• Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY Targeting of bone morphogenetic protein growth factor complexes to fibrillin. J. Biol. Chem., 2008-03-13;283(20):13874-88. 2008-03-13 [PMID: 18339631] (Western Blot, Human)
• Sato F, Kurokawa M, Yamauchi N, Hattori MA Gene silencing of myostatin in differentiation of chicken embryonic myoblasts by small interfering RNA. Am. J. Physiol., Cell Physiol., 2006-04-12;291(3):C538-45. 2006-04-12 [PMID: 16611734] (Western Blot, Chicken)

Bioinformatics

• Gene Symbol: GDF11
• Uniprot:
  • O08689()