GATA-6 Antibody (OTI2B3)

Western Blot: GATA-6 Antibody (OTI2B3) [NBP1-47766] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-GATA6 monoclonal antibody.(1:200)

Immunocytochemistry/Immunofluorescence: GATA-6 Antibody (OTI2B3) [NBP1-47766] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Gata6.

Immunohistochemistry-Paraffin: GATA-6 Antibody (OTI2B3) [NBP1-47766] - Staining of paraffin-embedded Human lung tissue using anti-Gata6 mouse monoclonal antibody.

Western Blot: GATA-6 Antibody (OTI2B3) [NBP1-47766] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Gata6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Gata6.

Immunocytochemistry/Immunofluorescence: GATA-6 Antibody (OTI2B3) [NBP1-47766] - Staining of HT29 cells using anti-Gata6 mouse monoclonal antibody.

Product Discontinued

GATA-6 Antibody (OTI2B3) Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 300-449 of human GATA6 (NP_005248) produced in E.coli.
• Specificity: This antibody is specific for Homo sapiens GATA binding protein 6 (GATA6).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GATA6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Theoretical MW: 59.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GATA-6 Antibody (OTI2B3)

• GATA binding protein 6
• GATA6
• GATA-6
• GATA-binding factor 6
• GATA-binding protein 6
• transcription factor GATA-6

Background

The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells (Tsai et al., 1994 [PubMed 8078582]). GATA6 Thought to be important for regulating terminal differentiation and/or proliferation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GATA-6 Antibody (NBP1-47766)(1)

Publication using NBP1-47766

• Peng T, Deng X, Tian F et al. The interaction of LOXL2 with GATA6 induces VEGFA expression and angiogenesis in cholangiocarcinoma Int. J. Oncol. 2019-07-15 [PMID: 31322171] (IHC-P, Human)

Bioinformatics

• Gene Symbol: GATA6
• Entrez:
  • 2627(Human)
• Uniprot:
  • Q92908()