GATA-6 Antibody (2F10G3) - BSA Free

Western Blot: GATA-6 Antibody (2F10G3) [NBP2-37377] - Western blot analysis using GATA6 mAb against HEK293 (1) and GATA6 (AA: 491-557)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: GATA-6 Antibody (2F10G3) [NBP2-37377] - Flow cytometric analysis of HEK293 cells using GATA6 mouse mAb (green) and negative control (red).

Western Blot: GATA-6 Antibody (2F10G3) [NBP2-37377] - Western blot analysis using GATA6 mAb against human GATA6 recombinant protein. (Expected MW is 32.3 kDa)

ELISA: GATA-6 Antibody (2F10G3) [NBP2-37377] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

GATA-6 Antibody (2F10G3) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human GATA6 (AA: 491-557) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GATA6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for GATA-6 Antibody (2F10G3) - BSA Free

• GATA binding protein 6
• GATA6
• GATA-6
• GATA-binding factor 6
• GATA-binding protein 6
• transcription factor GATA-6

Background

The Gata6 gene is a transcriptional activator that is crucial to modulating terminal differentiation and/or proliferation by regulating SEMA3C and PLXNA2. The Gata6 gene is most prominent during vertebrate development in early and later embryogenesis. Two isoforms exist: isoform 1 is 595 amino acids long at 60 kDa and isoform 2 exists at 449 amino acids in length and 45 kDA. The Gata6 gene focuses on endo- and mesodermally derived cells, thus participating significantly in gut, lung, and heart development and mutations of the Gata6 gene are linked to multiple congenital defects. The Gata6 gene has also been associated with polycystic ovary syndrome, hernias, teratoma, pulmonary fibrosis, germ cell tumor, gastric cancer, Crohn's disease, colorectal cancer, colon cancer, and carcinoma. It is known to interact with KLF13, MED1, HHEX, KLF2, and SP1 as it functions in pathways such as lymphocyte signaling, heart development, hemostasis, and thrombin signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA6