GATA-4 Antibody [Biotin]

NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." > NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." title="GATA-4 was detected in immersion fixed iBJ6, an induced pluripotent stem cell line, differentiated to endoderm using Goat Anti-Human GATA-4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2606) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." />

GATA-4 was detected in immersion fixed iBJ6, an induced pluripotent stem cell line, differentiated to endoderm using Goat Anti-Human GATA-4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2606) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # <a class=

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

GATA-4 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human GATA-4
  Met27-Phe211
  Accession # P43694
• Specificity: Detects human GATA‑4 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human GATA-1, -2, -3, -5, and -6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GATA4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-4 Antibody [Biotin]

• GATA binding protein 4
• GATA4
• GATA-4
• GATA-binding factor 4
• GATA-binding protein 4
• MGC126629
• transcription factor GATA-4

Background

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GATA-4 Antibody (BAF2606)(5)

Publications using BAF2606

• Moghe, P;Belousov, R;Ichikawa, T;Iwatani, C;Tsukiyama, T;Erzberger, A;Hiiragi, T; Coupling of cell shape, matrix and tissue dynamics ensures embryonic patterning robustness Nature cell biology 2025-02-18 [PMID: 39966670] (Immunohistochemistry, Transgenic Mouse)
• Tonin, R;Feo, F;Falliano, S;Giunti, L;Calamai, M;Procopio, E;Mari, F;Sciruicchio, V;Conti, V;Fanelli, I;Bambi, F;Guerrini, R;Morrone, A; Generation of a human induced pluripotent stem cell line from a patient with GM3 synthase deficiency using self-replicating RNA vector Stem cell research 2024-05-03 [PMID: 38703669] (Immunocytochemistry, Human)
• HK Ghayee, Y Xu, H Hatch, R Brockway, AS Multani, T Gu, WB Bollag, A Turcu, WE Rainey, J Rege, K Nanba, VJ Bhagwandin, F Nwariaku, V Stastny, AF Gazdar, JW Shay, RJ Auchus, SG Tevosian Development of Human Adrenocortical Adenoma (HAA1) Cell Line from Zona Reticularis International Journal of Molecular Sciences, 2022-12-29;24(1):. 2022-12-29 [PMID: 36614027] (ICC, Human)
• Allyson Quinn Ryan, Chii Jou Chan, François Graner, Takashi Hiiragi Lumen Expansion Facilitates Epiblast-Primitive Endoderm Fate Specification during Mouse Blastocyst Formation Developmental Cell 2019-12-16 [PMID: 31735667]
• Drukker M, Tang C, Ardehali R Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat. Biotechnol., 2012-05-27;30(6):531-42. 2012-05-27 [PMID: 22634564] (ICC, Human)

Bioinformatics

• Gene Symbol: GATA4
• Uniprot:
  • P43694()