GATA-4 Antibody (Evan)

Images

 
Western Blot: GATA-4 Antibody (Evan) [NBP1-43112] - Anti-Human/Mouse Gata-4 Purified (2 ug/ml) was used in a western blot of SDS lysates. Lane 1: NIH-3T3, Lane 2: C57Bl/6 mouse heart, Lane 3: BALB/c mouse heart.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
Evan
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

GATA-4 Antibody (Evan) Summary

Immunogen
The immunogen for this antibody was Gata4.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
GATA4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This Evan antibody has been tested by western blot analysis of mouse heart lysates. As a starting dilution, it is recommended to use this purified antibody at 2ug/ml for western blot analysis.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for GATA-4 Antibody (Evan)

  • GATA binding protein 4
  • GATA4
  • GATA-4
  • GATA-binding factor 4
  • GATA-binding protein 4
  • MGC126629
  • transcription factor GATA-4

Background

The Evan monoclonal antibody reacts with mouse and human Gata-4. In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights. The Evan monoclonal antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4. Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved DNA binding domain containing two zinc fingers. The mouse Gata-4 gene encodes a protein of 48kDa. Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival. Gata-4 is expressed during fetal development, as well as in the adult mouse heart. It also appears to modulate gene expression in the gut, liver, and gonads.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-16613
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31558
Species: Hu, Mu
Applications: WB, Flow, ICC/IF
NBP1-87992
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF1700
Species: Hu
Applications: WB, IHC, ChIP, ICC
PP-N1665-00
Species: Hu
Applications: WB, IHC, IP
NBP1-32895
Species: Hu, Mu, Rt
Applications: WB
NBP2-00493
Species: Hu, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF2170
Species: Hu
Applications: WB, ChIP, ICC
NBP2-14873
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF1700
Species: Hu
Applications: WB, IHC, ChIP, ICC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-83237
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2379
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
AF1997
Species: Hu
Applications: WB, ChIP, ICC
H00388112-B01P
Species: Hu
Applications: WB, ELISA
NBP1-87814
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
PPS046
Species: Hu, Mu, Rt, Bv, Ca, Ch, GP, Pm, Ze
Applications: WB

Publications for GATA-4 Antibody (NBP1-43112) (0)

There are no publications for GATA-4 Antibody (NBP1-43112).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GATA-4 Antibody (NBP1-43112) (0)

There are no reviews for GATA-4 Antibody (NBP1-43112). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GATA-4 Antibody (NBP1-43112) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GATA-4 Products

Bioinformatics Tool for GATA-4 Antibody (NBP1-43112)

Discover related pathways, diseases and genes to GATA-4 Antibody (NBP1-43112). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GATA-4 Antibody (NBP1-43112)

Discover more about diseases related to GATA-4 Antibody (NBP1-43112).
 

Pathways for GATA-4 Antibody (NBP1-43112)

View related products by pathway.

PTMs for GATA-4 Antibody (NBP1-43112)

Learn more about PTMs related to GATA-4 Antibody (NBP1-43112).
 

Research Areas for GATA-4 Antibody (NBP1-43112)

Find related products by research area.

Blogs on GATA-4

There are no specific blogs for GATA-4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GATA-4 Antibody (Evan) and receive a gift card or discount.

Bioinformatics

Gene Symbol GATA4
Entrez