GATA-4 Antibody (Evan) - BSA Free

Western Blot: GATA-4 Antibody (Evan) [NBP1-43112] - Anti-Human/Mouse Gata-4 Purified (2 ug/ml) was used in a western blot of SDS lysates. Lane 1: NIH-3T3, Lane 2: C57Bl/6 mouse heart, Lane 3: BALB/c mouse heart.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: Evan
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

GATA-4 Antibody (Evan) - BSA Free Summary

• Description: Novus Biologicals Rat GATA-4 Antibody (Evan) - BSA Free (NBP1-43112) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was Gata4.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: GATA4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: This Evan antibody has been tested by western blot analysis of mouse heart lysates. As a starting dilution, it is recommended to use this purified antibody at 2ug/ml for western blot analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for GATA-4 Antibody (Evan) - BSA Free

• GATA binding protein 4
• GATA4
• GATA-4
• GATA-binding factor 4
• GATA-binding protein 4
• MGC126629
• transcription factor GATA-4

Background

The Evan monoclonal antibody reacts with mouse and human Gata-4. In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights. The Evan monoclonal antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4. Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved DNA binding domain containing two zinc fingers. The mouse Gata-4 gene encodes a protein of 48kDa. Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival. Gata-4 is expressed during fetal development, as well as in the adult mouse heart. It also appears to modulate gene expression in the gut, liver, and gonads.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA4