GATA-4 Antibody (6H10)

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Western Blot: GATA4 Antibody (6H10) [NBP1-47540] - Western blot analysis using anti-GATA4 mAb against rat fetal heart (1) and adult heart (2) tissues lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clone
6H10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GATA-4 Antibody (6H10) Summary

Immunogen
Purified recombinant fragment of human GATA4 expressed in E. coli. [UniProt# P43694]
Localization
Nucleus.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GATA4
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
Application Notes
This GATA4 (6H10) antibody is useful for Western blot and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-47540 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for GATA-4 Antibody (6H10)

  • GATA binding protein 4
  • GATA4
  • GATA-4
  • GATA-binding factor 4
  • GATA-binding protein 4
  • MGC126629
  • transcription factor GATA-4

Background

GATA4, also known as GATA binding protein 4, is a 46 kDa member of the GATA family of zinc-finger transcription factors. Members of this family is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. These factors recognize the GATA motif which is present in the promoters of many genes. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-4 Antibody (NBP1-47540)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Filter By Species
Mouse
(1)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GATA4
Entrez
Uniprot