GATA-4 Antibody (6H10) - BSA Free

Western Blot: GATA4 Antibody (6H10) [NBP1-47540] - Western blot analysis using anti-GATA4 mAb against rat fetal heart (1) and adult heart (2) tissues lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clone: 6H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

GATA-4 Antibody (6H10) - BSA Free Summary

• Description: Novus Biologicals Mouse GATA-4 Antibody (6H10) - BSA Free (NBP1-47540) is a monoclonal antibody validated for use in WB and ELISA. Anti-GATA-4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human GATA4 expressed in E. coli. [UniProt# P43694]
• Localization: Nucleus.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GATA4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:500-1:2000
• Application Notes: This GATA4 (6H10) antibody is useful for Western blot and ELISA.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for GATA-4 Antibody (6H10) - BSA Free

• GATA binding protein 4
• GATA4
• GATA-4
• GATA-binding factor 4
• GATA-binding protein 4
• MGC126629
• transcription factor GATA-4

Background

GATA4, also known as GATA binding protein 4, is a 46 kDa member of the GATA family of zinc-finger transcription factors. Members of this family is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. These factors recognize the GATA motif which is present in the promoters of many genes. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GATA-4 Antibody (NBP1-47540)(1)

Publication using NBP1-47540

• Sato T, Okumura F, Ariga T et al. TRIM6 interacts with Myc and maintains the pluripotency of mouse embryonic stem cells. J Cell Sci. 2012-03-15 [PMID: 22328504] (WB, Mouse)

Bioinformatics

• Gene Symbol: GATA4
• Entrez:
  • 2626(Human)
  • 14463(Mouse)
• Uniprot:
  • P43694()