GATA-4 Antibody (532020) [Unconjugated]

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue . PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human GATA‑4 Monoclonal Antibody (Catalog # MAB2606) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
532020
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

GATA-4 Antibody (532020) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-4
Met27-Phe211
Accession # P43694
Specificity
Detects human GATA-4 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GATA4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB2606 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-4 Antibody (532020) [Unconjugated]

  • GATA binding protein 4
  • GATA4
  • GATA-4
  • GATA-binding factor 4
  • GATA-binding protein 4
  • MGC126629
  • transcription factor GATA-4

Background

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. GATA-4 contains 2 zinc finger-binding domains and migrates at 56 kDa in SDS-PAGE gels. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-4 Antibody (MAB2606)(2)

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GATA4
Uniprot