GATA-4 Antibody (532020) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clone: 532020
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

GATA-4 Antibody (532020) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human GATA-4
  Met27-Phe211
  Accession # P43694
• Specificity: Detects human GATA-4 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-4 Antibody (532020) [Alexa Fluor® 647]

• GATA binding protein 4
• GATA4
• GATA-4
• GATA-binding factor 4
• GATA-binding protein 4
• MGC126629
• transcription factor GATA-4

Background

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. GATA-4 contains 2 zinc finger-binding domains and migrates at 56 kDa in SDS-PAGE gels. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.