GATA-3 Antibody

Western Blot: GATA-3 Antibody [NBP1-51912] - Ataining of Human Tonsil (A) and Mouse Kidney (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GATA-3 Antibody Summary

• Description: Novus Biologicals Goat GATA-3 Antibody (NBP1-51912) is a polyclonal antibody validated for use in WB and ELISA. Anti-GATA-3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence EVTADQPRWVSHH-C corresponding to N-Terminus according to NP_001002295.1, NP_002042.1.
• Epitope: EVTADQPRWVSHH-C
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GATA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 47 kDa band observed in human tonsil, thymus, and human breast cancer lysates (calculated MW of 48 kDa band according to NP_001002295.1 and of 47.9 kDa band according to NP_002042.1). An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide. have any such results been reported with other antibodies/lysates?

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GATA-3 Antibody

• 131320
• GATA binding protein 3
• GATA3
• GATA-3
• GATA-binding factor 3
• GATA-binding protein 3
• HDRMGC5199
• MGC2346
• MGC5445
• trans-acting T-cell-specific transcription factor GATA-3

Background

GATA3 is a 45-50 kD transcription factor found in the nucleus of T cells, mast cells, and embryonic brain and kidney that contains a GATA-type zinc finger motif. This transcription factor recognizes and binds the GATA core nucleotide motif (A/T) GATA (A/G) and has been shown to regulate T-H1 and T-H2 cell differentiation by directly binding to the IL-5 and IL-13 promoters, remodeling chromatin structure to open the IL-4 locus, and repressing IL-12R beta-2 chain expression. GATA3 has been shown to be induced by IL-4 through Stat6 and can be upregulated by positive feedback. GATA-3 can be modified by acetylation and has been shown to interact with Smad3. The Poly6071 antibody has been shown to be useful for Western blotting of human GATA3. Smad3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GATA-3 Antibody (NBP1-51912)(1)

Publication using NBP1-51912

• Marine J, Winoto A. The human enhancer-binding protein Gata3 binds to several T-cell receptor regulatory elements. Proc Natl Acad Sci U S A 1991-08-15 [PMID: 1871134]

Bioinformatics

• Gene Symbol: GATA3
• Entrez:
  • 2625(Human)
  • 14462(Mouse)
• Uniprot:
  • NP_001002295.1(Human)