GATA-3 Antibody

Images

 
Western Blot: GATA-3 Antibody [NBP1-51912] - Ataining of Human Tonsil (A) and Mouse Kidney (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: GATA-3 Antibody [NBP1-51912] - (3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) staining of cells in the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GATA-3 Antibody Summary

Immunogen
Peptide with sequence EVTADQPRWVSHH-C corresponding to N-Terminus according to NP_001002295.1, NP_002042.1.
Epitope
EVTADQPRWVSHH-C
Specificity
This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 3 ug/ml
  • Immunohistochemistry-Paraffin 3 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 47 kDa band observed in human tonsil, thymus, and human breast cancer lysates (calculated MW of 48 kDa band according to NP_001002295.1 and of 47.9 kDa band according to NP_002042.1). An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide. have any such results been reported with other antibodies/lysates? IHC-P: Human tonsil stains nuclei of some cells in the interfollicular area and in germinal centres. Data obtained with a previous batch (different goat).
Publications
Read Publication using NBP1-51912.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GATA-3 Antibody

  • 131320
  • GATA binding protein 3
  • GATA3
  • GATA-3
  • GATA-binding factor 3
  • GATA-binding protein 3
  • HDRMGC5199
  • MGC2346
  • MGC5445
  • trans-acting T-cell-specific transcription factor GATA-3

Background

GATA3 is a 45-50 kD transcription factor found in the nucleus of T cells, mast cells, and embryonic brain and kidney that contains a GATA-type zinc finger motif. This transcription factor recognizes and binds the GATA core nucleotide motif (A/T) GATA (A/G) and has been shown to regulate T-H1 and T-H2 cell differentiation by directly binding to the IL-5 and IL-13 promoters, remodeling chromatin structure to open the IL-4 locus, and repressing IL-12R beta-2 chain expression. GATA3 has been shown to be induced by IL-4 through Stat6 and can be upregulated by positive feedback. GATA-3 can be modified by acetylation and has been shown to interact with Smad3. The Poly6071 antibody has been shown to be useful for Western blotting of human GATA3. Smad3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-3 Antibody (NBP1-51912)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GATA3
Entrez
Uniprot