GATA-3 Antibody


Western Blot: GATA-3 Antibody [NBP1-51912] - Ataining of Human Tonsil (A) and Mouse Kidney (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: GATA-3 Antibody [NBP1-51912] - (3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) staining of cells in the more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
0.5 mg/ml

Order Details

GATA-3 Antibody Summary

Peptide with sequence EVTADQPRWVSHH-C corresponding to N-Terminus according to NP_001002295.1, NP_002042.1.
This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris saline, pH 7.3 with 0.5% BSA.
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified


Application Notes
WB: Approx. 47 kDa band observed in human tonsil, thymus, and human breast cancer lysates (calculated MW of 48 kDa band according to NP_001002295.1 and of 47.9 kDa band according to NP_002042.1). An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide. have any such results been reported with other antibodies/lysates? IHC-P: Human tonsil stains nuclei of some cells in the interfollicular area and in germinal centres. Data obtained with a previous batch (different goat).
Read Publication using NBP1-51912.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.


This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).

Alternate Names for GATA-3 Antibody

  • 131320
  • GATA binding protein 3
  • GATA3
  • GATA-binding factor 3
  • GATA-binding protein 3
  • HDRMGC5199
  • MGC2346
  • MGC5445
  • trans-acting T-cell-specific transcription factor GATA-3


GATA3 is a 45-50 kD transcription factor found in the nucleus of T cells, mast cells, and embryonic brain and kidney that contains a GATA-type zinc finger motif. This transcription factor recognizes and binds the GATA core nucleotide motif (A/T) GATA (A/G) and has been shown to regulate T-H1 and T-H2 cell differentiation by directly binding to the IL-5 and IL-13 promoters, remodeling chromatin structure to open the IL-4 locus, and repressing IL-12R beta-2 chain expression. GATA3 has been shown to be induced by IL-4 through Stat6 and can be upregulated by positive feedback. GATA-3 can be modified by acetylation and has been shown to interact with Smad3. The Poly6071 antibody has been shown to be useful for Western blotting of human GATA3. Smad3


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, Simple Western, IHC, ICC
Species: Hu, Mu
Applications: ELISA(Cap), ELISA(Cap), ELISA(Det), ELISA(Det), Neut, ELISA(Sta), ELISA(Sta)
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC
Species: Hu, Mu
Applications: Flow, IHC, ICC
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, InhibTFunc
Species: Ch, Vi
Applications: WB, ELISA, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA

Publications for GATA-3 Antibody (NBP1-51912)(1)

Reviews for GATA-3 Antibody (NBP1-51912) (0)

There are no reviews for GATA-3 Antibody (NBP1-51912). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GATA-3 Antibody (NBP1-51912) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional GATA-3 Antibody Products

Related Products by Gene

Bioinformatics Tool for GATA-3 Antibody (NBP1-51912)

Discover related pathways, diseases and genes to GATA-3 Antibody (NBP1-51912). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GATA-3 Antibody (NBP1-51912)

Discover more about diseases related to GATA-3 Antibody (NBP1-51912).

Pathways for GATA-3 Antibody (NBP1-51912)

View related products by pathway.

PTMs for GATA-3 Antibody (NBP1-51912)

Learn more about PTMs related to GATA-3 Antibody (NBP1-51912).

Research Areas for GATA-3 Antibody (NBP1-51912)

Find related products by research area.

Blogs on GATA-3

There are no specific blogs for GATA-3, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol GATA3

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-51912 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought