GATA-2 Antibody - BSA Free

Western Blot: GATA-2 Antibody [NBP2-16612] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: GATA-2 Antibody [NBP2-16612] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Frozen: GATA-2 Antibody [NBP2-16612] - Frozen-sectioned rat E13.5 brain. Green: GATA2 stained by GATA2 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GATA-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GATA-2 Antibody - BSA Free (NBP2-16612) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GATA-2. The exact sequence is proprietary.
• Predicted Species: Porcine (99%), Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GATA2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (86%), Xenopus laevis (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GATA-2 Antibody - BSA Free

• endothelial transcription factor GATA-2
• FLJ45948
• GATA binding protein 2
• GATA2
• GATA-2
• GATA-binding protein 2NFE1B
• MGC2306

Background

This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GATA2
• Uniprot:
  • P23769()