GARS Antibody (5Q5D7)

Western Blot: GARS Antibody (5Q5D7) [NBP3-15293] - Western blot analysis of Jurkat, using GARS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.

Western Blot: GARS Antibody (5Q5D7) [NBP3-15293] - Western blot analysis of extracts from wild type(WT) and GARS knockout (KO) 293T cells, using GARS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.

Western Blot: GARS Antibody (5Q5D7) [NBP3-15293] - Western blot analysis of various lysates, using [KO Validated] GARS Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 180s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 5Q5D7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GARS Antibody (5Q5D7) Summary

• Description: Novus Biologicals Rabbit GARS Antibody (5Q5D7) (NBP3-15293) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 640-739 of human GARS (P41250). RHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GARS1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GARS Antibody (5Q5D7)

• AP-4-A synthetase
• Charcot-Marie-Tooth neuropathy 2D
• Charcot-Marie-Tooth neuropathy, neuronal type, D
• Diadenosine tetraphosphate synthetase
• DSMAV
• glycine tRNA ligase
• Glycine--tRNA ligase
• glycyl-tRNA synthetase
• GlyRSEC 6.1.1.14
• HMN5
• SMAD1CMT2D

Background

GARS encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GARS1