Galectin-1 Antibody [Biotin]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1152) followed by Streptavidin-HRP. A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Galectin-1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Galectin‑1 (R&D Systems, Catalog # 1152-GA)
  Ala2-Asp135
  Accession # P09382
• Specificity: Detects human Galectin-1 in Western blots. In this format, approximately 20% cross-reactivity with recombinant mouse Galectin‑1 is observed and less than 5% cross-reactivity with recombinant human (rh) Galectin-2, rhGalectin-4, rhGalectin-7, and rhGalectin-8 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LGALS1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-1 Antibody [Biotin]

• 14 kDa laminin-binding protein
• 14 kDa lectin
• Beta-galactoside-binding lectin L-14-I
• BHL
• DKFZp686E23103
• GAL1
• gal-1
• Galaptin
• galectin 1
• Galectin1
• Galectin-1
• GBP
• HBL
• HLBP14
• HPL
• L-14
• Lactose-binding lectin 1
• Lectin galactoside-binding soluble 1
• lectin, galactoside-binding, soluble, 1
• LGALS1
• Putative MAPK-activating protein PM12
• S-Lac lectin 1

Background

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Galectin-1, also known as L-14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3 and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Human and mouse galectin-1 share about 88% amino acid sequence similarity (1-4).

1. Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
3. Hughes, R.C. (2001) Biochimie 83:667.
4. R&D Systems’ Cytokine Bulletin; Summer 2002.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galectin-1 Antibody (BAF1152)(5)

Publications using BAF1152

• HM Hsu, YY Yang, YH Huang, CH Chu, TJ Tu, YT Wu, CJ Chiang, SB Yang, DK Hsu, FT Liu, JH Tai Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates PloS Neglected Tropical Diseases, 2023-01-03;17(1):e0011016. 2023-01-03 [PMID: 36595499] (ELISA Detection, Human)
• Tanja Freichel, Viktoria Heine, Dominic Laaf, Eleanor E. Mackintosh, Sophia Sarafova, Lothar Elling et al. Sequence‐Defined Heteromultivalent Precision Glycomacromolecules Bearing Sulfonated/Sulfated Nonglycosidic Moieties Preferentially Bind Galectin‐3 and Delay Wound Healing of a Galectin‐3 Positive Tumor Cell Line in an In Vitro Wound Scratch Assay Macromolecular Bioscience 2020-09-01 [PMID: 32715650]
• MN Andersen, M Ludvigsen, N Abildgaard, I Petruskevi, R Hjortebjer, M Bjerre, B Honoré, HJ Møller, NF Andersen Serum galectin-1 in patients with multiple myeloma: associations with survival, angiogenesis, and biomarkers of macrophage activation Onco Targets Ther, 2017-04-04;10(0):1977-1982. 2017-04-04 [PMID: 28435287] (ELISA Development (Detection), Human)
• Ga Bin Park, Yoon Hee Chung, Daejin Kim Induction of galectin-1 by TLR-dependent PI3K activation enhances epithelial-mesenchymal transition of metastatic ovarian cancer cells Oncology Reports 2017-05-01 [PMID: 28350104]
• Sioud M, Mobergslien A, Boudabous A, Floisand Y Mesenchymal stem cell-mediated T cell suppression occurs through secreted galectins. Int. J. Oncol., 2010-12-09;38(2):385-90. 2010-12-09 [PMID: 21165555] (Flow Cytometry, Western Blot, Human)

Bioinformatics

• Gene Symbol: LGALS1
• Uniprot:
  • P09382()