Galectin-10 Antibody [Unconjugated]

Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5447) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Human Tregs were expanded from PBMC using Cloudz Human Treg Expansion Kit (CLD006) then stained with (A) Goat Anti-Human Galectin-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5447) or (B) Goat IgG Control Antibody (AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107) and Mouse Anti-Human CD25/IL-2 Ra Allophycocyanin-conjugated Monoclonal Antibody (FAB1020A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Human PBMC lymphocytes were stained with Goat Anti-Human Galectin‑10 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5447) followed by CFS-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109) and Mouse Anti-Human CD25/IL-2 R alpha Allophycocyanin-conjugated Monoclonal Antibody (Catalog # FAB1020A).Quadrant markers were set based on control antibody staining (Catalog # AB‑108‑C).

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Galectin-10 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Galectin‑10
  Ser2-Arg142
  Accession # Q05315
• Specificity: Detects human Galectin-10 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-10 Antibody [Unconjugated]

• Charcot-Leyden crystal proteinLGALS10A
• CLC
• EC 3.1.1.5
• eosinophil lysophospholipase
• GAL10
• Gal-10
• galectin 10
• Galectin10
• Galectin-10
• LGALS10
• LPPL_HUMAN
• Lysolecithin acylhydrolase
• MGC149659

Background

Galectin-10 (also eosinophil lysophospholipase and Charcot-Leyden Crystal protein) is a 16 kDa member of the lectin family of proteins. It is expressed intracellularly by eosinophils, basophils and CD25+ Treg cells. Although originally believed to possess lysophospholipase activity, this has been shown to be incorrect. It is known to bind lysophospholipase and its inhibitors and to bind mannose in a very unusual manner. Human Galectin-10 is 142 amino acids (aa) in length. There is one Galectin domain (aa 6‑138) that contains two dimerization motifs (aa 6‑10 and 131‑135). Two molecular weight isoforms of 15 and 14 kDa have been described that are not yet characterized. There is no known structural rodent counterpart to human Galectin-10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galectin-10 Antibody (AF5447)(1)

Publication using AF5447

• Chua , Justin C, Douglass , Jo A, Gillman , Andrew Galectin-10, a potential biomarker of eosinophilic airway inflammation. PLoS ONE, 2012-08-06;7(8):e42549. 2012-08-06 [PMID: 22880030] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CLC
• Uniprot:
  • Q05315()