Galanin R2/GALR2 Antibody

Western Blot: Galanin R2/GALR2 Antibody [NB100-57081] - (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Galanin R2/GALR2 Antibody Summary

• Immunogen: Peptide with sequence C-RYPLHSRELRTPRN corresponding to internal region according to NP_003848.1.
• Epitope: RYPLHSRELRTPRN
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GALR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human heart lysates (calculated MW of 41.7 kDa band according to NP_003848.1).
• Control:

  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Galanin R2/GALR2 Antibody

• GAL2R
• GAL2-R
• Galanin R2
• galanin receptor 2
• galanin receptor type 2
• GalaninR2
• GALNR2
• GALNR2MGC125984
• GALR2
• GALR-2
• MGC125983

Background

Galanin is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7-transmembrane family of G protein-coupled receptors. GALR2 interacts with the N-terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galanin R2/GALR2 Antibody (NB100-57081)(1)

Publication using NB100-57081

• Berger A, Tuechler C, Almer D et al. Elevated expression of galanin receptors in childhood neuroblastic tumors. Neuroendocrinology 2002-02-01 [PMID: 11867941]

Bioinformatics

• Gene Symbol: GALR2
• Entrez:
  • 8811(Human)
  • 14428(Mouse)
  • 29234(Rat)
• Uniprot:
  • NP_003848.1(Human)