Galanin R3/GALR3 Antibody

Western Blot: Galanin R3/GALR3 Antibody [NB100-2846] - (2ug/ml) Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Galanin R3/GALR3 Antibody [NB100-2846] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Galanin R3/GALR3 Antibody Summary

• Immunogen: Peptide with sequence C-QGPEPREGPVHGGE corresponding to C-Terminus according to NP_003605.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GALR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in human skin and human testis lysates (calculated MW of 39.6 kDa band according to NP_003605.1). IHC-P: Human testis shows cellular membrane staining of spermatocytes.
• Control:

  Human Skin Whole Tissue Lysate (Adult Whole Normal)
  Galanin R3/GALR3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Galanin R3/GALR3 Antibody

• GAL3-R
• Galanin R3
• galanin receptor 3
• galanin receptor type 3
• galanin receptor, family member 3
• GalaninR3
• GALNR3
• GALR3
• GALR-3

Background

GalR3 is a Galanin Receptor that is similar, but distinct from the other galanin receptors, and it mediates the effects of the galanin peptide in a wide variety of tissues. Like GalR1, the signaling mechanism is predominantly through inhibition of adenylate cyclase. GalR3 has been reported in brain, testis, adrenal gland, and pancreas. An EST has been isolated from an ovarian thecoma fibroma library.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galanin R3/GALR3 Antibody (NB100-2846)(1)

Publication using NB100-2846

• Swanson CJ, Blackburn TP, Zhang X et al. Anxiolytic- and antidepressant-like profiles of the galanin-3 receptor (Gal3) antagonists SNAP 37889 and SNAP 398299. Proc Natl Acad Sci U S A 2005-11-29 [PMID: 16287967]

Bioinformatics

• Gene Symbol: GALR3
• Entrez:
  • 8484(Human)
• Uniprot:
  • NP_003605.1(Human)