GABA-AR alpha 4 Antibody - Azide Free

Western Blot: GABA-A R alpha 4 Antibody [NB300-193] - Rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~64k a4-subunit of the GABA-A R.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

GABA-AR alpha 4 Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit GABA-AR alpha 4 Antibody - Azide Free (NB300-193) is a polyclonal antibody validated for use in IHC and WB. Anti-GABA-AR alpha 4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein from the cytoplasmic loop of the alpha 4 subunit. Accession # P28471
• Localization: Integral membrane protein.
• Specificity: Specific for endogenous levels of the ~ 64 kDa alpha 4-subunit of the GABAA receptor.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABRA4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:300
  • Western Blot 1:1000
• Application Notes: NB300-193 may be used for Western blot analysis where a band is seen around 64 kDa.
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for GABA-AR alpha 4 Antibody - Azide Free

• GABA A R alpha 4
• GABA(A) receptor subunit alpha-4
• GABAAR alpha 4
• GABAARa4
• GABRA4
• gamma-aminobutyric acid (GABA) A receptor, alpha 4
• gamma-aminobutyric acid receptor subunit alpha-4

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 4 Antibody (NB300-193)(2)

Publications using NB300-193

• Marutha Ravindran CR, Mehta AK, Ticku MK. Effect of chronic administration of ethanol on the regulation of tyrosine kinase phosphorylation of the GABAA receptor subunits in the rat brain. Neurochem Res 2007-07-01 [PMID: 17404840] (WB, Rat)
• Werner DF. Elucidating the Role of Alpha1-containing GABA(A) Receptors in Ethanol Action PhD Thesis University of Pittsburgh (ISBN 0549455426, 9780549455424). 2007-01-01 (WB, Mouse)

FAQs for GABA-AR alpha 4 Antibody (NB300-193). (Showing 1 - 1 of 1 FAQs).

1. I am looking for a Rabbit GABA A Receptor alpha 4 Polyclonal Antibody that reacts with rat. I found NB300-193 with the Immunogen as "Fusion protein from the cytoplasmic loop of the alpha 4-subunit of rat GABAA receptor". Whereas with other suppliers I got the same product with Immunogen as " Fusion protein of MBP with the amino acid sequence from the cytosolic loop of rat GABA-A receptor alpha 4 subunit." I am not sure whether your product's Immunogen has Fusion protein of MBP or without?
   • Yes, the fusion protein is MBP. You may also be interested in NB300-194, as this is another rabbit anti-rat polyclonal antibody for GABA A receptor alpha 4.

Bioinformatics

• Gene Symbol: GABRA4
• Entrez:
  • 2557(Human)
  • 140675(Rat)
• Uniprot:
  • P28471(Rat)