GABA-A R beta 1 Antibody (S96-55) - BSA Free

Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, MA
• Clone: S96-55
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GABA-A R beta 1 Antibody (S96-55) - BSA Free Summary

• Immunogen: Fusion protein amino acids 327-450 of mouse GABA-A-R-Beta 1
• Localization: Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
• Specificity: Detects approx 55kDa. No cross-reactivity against GABA-A-R-Beta 2 or -Beta3.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GABRB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.1-1.0 ug/ml
  • Immunohistochemistry 1:1000
  • Microarray
  • Western Blot 1-10 ug/ml
• Application Notes: 2 ug/ml of GABA-A Receptor Beta1 Antibody was sufficient for detection of Beta1 GABA receptor in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28197552).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for GABA-A R beta 1 Antibody (S96-55) - BSA Free

• GABA A R beta 1
• GABA(A) receptor subunit beta-1
• GABAAR beta 1
• GABAARb1
• GABRB1
• gamma-aminobutyric acid (GABA) A receptor, beta 1
• gamma-aminobutyric acid receptor subunit beta-1

Background

GABA A Receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA A Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-A R beta 1 Antibody (NBP1-48319)(2)

Publications using NBP1-48319

• Hernandez CC, Zhang Y, Hu N et al. GABA A Receptor Coupling Junction and Pore GABRB3 Mutations are Linked to Early-Onset Epileptic Encephalopathy Sci Rep. 2017-11-21 [PMID: 29162865] (WB, Human)
• Hernandez CC, Kong W, Hu N et al. Altered Channel Conductance States and Gating of GABAA Receptors by a Pore Mutation Linked to Dravet Syndrome. eNeuro. 2017-02-15 [PMID: 28197552] (WB, Human)

Bioinformatics

• Gene Symbol: GABRB1
• Uniprot:
  • P50571(Human)