GABA-AR alpha 1 Antibody - Azide Free

Western Blot: GABA-AR alpha 1 Antibody [NB300-207] - Mouse forebrain lysates from wild type (Control) and a1-knockout (a1-K/O) animals showing specific immunolabeling of the ~51k a1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a1-knockout animals.

• Reactivity: Mu, Rt, Hu, Bv, Ca, Pm
• Applications: WB, IHC, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

GABA-AR alpha 1 Antibody - Azide Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit GABA-AR alpha 1 Antibody - Azide Free (NB300-207) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-GABA-AR alpha 1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein from the cytoplasmic loop of the alpha 1 subunit. Accession # P62813
• Localization: Membrane; multi pass membrane protein.
• Specificity: Specific for endogenous levels of the ~51 kDa alpha1-subunit of the GABAA receptor. Labeling is absent in alpha1-subunit knockout animals.
• Predicted Species: Human (100%), Canine (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABRA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot 1:1000
• Application Notes: Use in immunoprecipitation reported in scientific literature (PMID:28536106).
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

It is anticipated that the antibody will react with bovine, canine, human, and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for GABA-AR alpha 1 Antibody - Azide Free

• ECA4
• EJM
• EJM5GABA(A) receptor subunit alpha-1
• GABA A R alpha 1
• GABAAR alpha 1
• GABAARa1
• GABRA1
• gamma-aminobutyric acid (GABA) A receptor, alpha 1
• gamma-aminobutyric acid receptor subunit alpha-1

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 1 Antibody (NB300-207)(2)

Publications using NB300-207

• Lindemeyer AK, Shen Y, Yazdani F et al. alpha 2 Subunit-Containing GABAA Receptor Subtypes Are Upregulated and Contribute to Alcohol-Induced Functional Plasticity in the Rat Hippocampus Mol Pharmacol. 2018-07-31 [PMID: 28536106] (WB, IP, Mouse)
• Roberts, D S, Hu, Y, Lund, I V, Brooks-Kayal, A R, Russek, S J. Brain-derived Neurotrophic Factor (BDNF)-induced Synthesis of Early Growth Response Factor 3 (Egr3) Controls the Levels of Type A GABA Receptor{alpha}4 Subunits in Hippocampal Neurons. 281, 29431-29435. 2006-01-01 [PMID: 16901909] (WB, Rat)

Bioinformatics

• Gene Symbol: GABRA1
• Entrez:
  • 2554(Human)
  • 29705(Rat)
• Uniprot:
  • P62813(Rat)