GABA-AR alpha 3 Antibody

Images

 
Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499] - Analysis in human cell line U-87 MG.
Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse ventral pallidum shows strong labeling of synaptic fields.
Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse olfactory bulb shows labelling of both neurons and synapses.
Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse insular cortex shows strong immunoreactivity in synapses.
Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse thalamus shows strong positivity in the paraventricular thalamic nucleus.
Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of human cerebral cortex shows moderate positivity in neuronal processes in neuropil.
Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GABA-AR alpha 3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TSHCYMTSLGILFLINILPGTTGQGESRRQEPGDFVKQDIGGLSPKHAPDIPDDSTDNITIFTRILDRLLDGYDNRLRPGLGDAVTEVKTDIYVTSFGPVSDTDMEYTIDVFFRQTWHDE
Predicted Species
Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GABRA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
GABA-AR alpha 3 Recombinant Protein Antigen (NBP1-87499PEP)
Publications
Read Publications using
NBP1-87499 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GABA-AR alpha 3 Antibody

  • GABA A R alpha 3
  • GABA(A) receptor subunit alpha-3
  • GABAARa3
  • GABRA3
  • gamma-aminobutyric acid (GABA) A receptor, alpha 3
  • gamma-aminobutyric acid receptor subunit alpha-3
  • gamma-animobutyric acid (GABA) A receptor, alpha 3
  • MGC33793

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; P ltl et al., 2003).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 3 Antibody (NBP1-87499)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GABRA3
Entrez
Uniprot