![Western Blot: EFHC1 Antibody [H00114327-D01P] - Analysis of EFHC1 expression in mouse testis.](http://images.novusbio.com/fullsize2/EFHC1-Antibody-Western-Blot-H00114327-D01P-img0003.jpg "Western Blot: EFHC1 Antibody [H00114327-D01P] - Analysis of EFHC1 expression in mouse testis.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | EFHC1 (AAH20210.1, 1 a.a. ~ 640 a.a) full-length human protein.MVSNPVHGLPFLPGTSFKDSTKTAFHRSQTLSYRNGYAIVRRPTVGIGGDRLQFNQLSQAELDELASKAPVLTYGQPKQAPPADFIPAHVAFDKKVLKFDAYFQEDVPMSTEEQYRIRQVNIYYYLEDDSMSVIEPVVENSGILQGKLIKRQRLAKNDRGDHYHWKDLNRGINITIYGKTFRVVDCDQFTQVFLESQGIELNPPEKMALDPYTELRKQPLRKYVTPSDFDQLKQFLTFDKQVLRFYAIWDDTDSM |
| Specificity | Reacts with EF-hand domain (C-terminal) containing 1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EFHC1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is reactive against tissue and transfected lysate in western blot, and as a detection antibody in ELISA. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | Protein A purified |
| Publication using H00114327-D01P | Applications | Species |
|---|---|---|
| Raju PK, Satishchandra P, Nayak S et al. Microtubule-associated defects caused by EFHC1 mutations in juvenile myoclonic epilepsy. Hum Mutat 2017 Mar 31 [PMID: 28370826] |
Secondary Antibodies |
Isotype Controls |
Diseases for EFHC1 Antibody (H00114327-D01P)Discover more about diseases related to EFHC1 Antibody (H00114327-D01P).
| Pathways for EFHC1 Antibody (H00114327-D01P)View related products by pathway.
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| Gene Symbol | EFHC1 |
| Entrez |
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| Uniprot |
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![Western Blot: EFHC1 Antibody [H00114327-D01P] - Analysis of EFHC1 expression in transfected 293T cell line by EFHC1 polyclonal antibody.Lane 1: EFHC1 transfected lysate(73.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/EFHC1 Antibody-Western Blot-H00114327-D01P-img0004.jpg "Western Blot: EFHC1 Antibody [H00114327-D01P] - Analysis of EFHC1 expression in transfected 293T cell line by EFHC1 polyclonal antibody.Lane 1: EFHC1 transfected lysate(73.90 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: GABA-A R alpha 1 Antibody [NB300-191] - Mouse forebrain lysates from Wild Type (WT) and alpha 1-knockout (K/O) animals showing specific immunolabeling of the -51 kDa alpha 1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 1-knockout animals.](http://images.novusbio.com/fullsize2/GABA-A-R-alpha-1-Antibody-Western-Blot-NB300-191-img0003.jpg)
![Western Blot: BRD2 Antibody [NBP1-84310] - Analysis in human cell lines HEK293 and MCF-7 using Anti-BRD2 antibody. Corresponding BRD2 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize2/BRD2-Antibody-Western-Blot-NBP1-84310-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-84310] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.](http://images.novusbio.com/fullsize2/BRD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84310-img0004.jpg)
![Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-84310] - Staining in human testis and liver tissues. Corresponding BRD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/BRD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84310-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CLCN2 Antibody [NBP1-85585] - Staining of human cell line CACO-2 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/CLCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85585-img0005.jpg)
![Immunohistochemistry-Paraffin: CLCN2 Antibody [NBP1-85585] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize2/CLCN2 Antibody-Immunohistochemistry-Paraffin-NBP1-85585-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Scn1a Antibody [NBP2-58876] - Staining of human cell line ASC TERT1 shows localization to nucleoplasm & plasma membrane.](http://images.novusbio.com/fullsize2/Scn1a Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58876-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Protocadherin beta 11 Antibody [NBP2-13738] - Staining of human cell line RH-30 shows localization to plasma membrane.](http://images.novusbio.com/fullsize2/Protocadherin-beta-11-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13738-img0002.jpg)
![Immunohistochemistry-Paraffin: Protocadherin beta 11 Antibody [NBP2-13738] - Staining of human prostate shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize2/Protocadherin-beta-11-Antibody-Immunohistochemistry-Paraffin-NBP2-13738-img0001.jpg)
![Western Blot: ME2 Antibody [NBP1-89559] - Analysis in human cell line Daudi.](http://images.novusbio.com/fullsize2/ME2-Antibody-Western-Blot-NBP1-89559-img0010.jpg)
![Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining in human duodenum and liver tissues using anti-ME2 antibody. Corresponding ME2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/ME2-Antibody-Immunohistochemistry-Paraffin-NBP1-89559-img0009.jpg)
![Western Blot: ME2 Antibody [NBP1-89559] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/ME2-Antibody-Western-Blot-NBP1-89559-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CELSR1 Antibody [NBP2-55658] - Staining of human cell line A549 shows localization to plasma membrane.](http://images.novusbio.com/fullsize2/CELSR1 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55658-img0001.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Mouse whole brain lysate showing specific immunolabeling of the ~46 kDa gamma-2-subunit of GABAa -R.](http://images.novusbio.com/fullsize2/GABA-A-R-gamma-2-Antibody-Western-Blot-NB300-190-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - Immunostaining of mouse retina showing specific labeling of the GABAa gamma-2 subunit in green, calbindin in red and DNA in blue. Photo courtesy of Dr. Arlene Hirano, UCLA.](http://images.novusbio.com/fullsize2/GABA-A-R-gamma-2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-190-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Kv7.2 Antibody [NBP2-38820] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize2/Kv7.2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38820-img0003.jpg)
![Immunohistochemistry-Paraffin: Kv7.2 Antibody [NBP2-38820] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize2/Kv7.2-Antibody-Immunohistochemistry-Paraffin-NBP2-38820-img0005.jpg)
![Immunohistochemistry: Kv7.2 Antibody [NBP2-38820] - Staining of human hippocampus shows distinct nuclear positivity in glial cells.](http://images.novusbio.com/fullsize2/Kv7.2-Antibody-Immunohistochemistry-NBP2-38820-img0002.jpg)
![Western Blot: Kv7.3 Antibody [NBP1-74102] - Lanes: 100 ug CHO cell lysate Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Goat anti-rabbit HRP Secondary Antibody Dilution: 1 : 25000 Gene name: Kcnq3 Submitted by: Anonymous.](http://images.novusbio.com/fullsize2/Kv7.3 Antibody-Western Blot-NBP1-74102-img0004.jpg)
![Western Blot: Kv7.3 Antibody [NBP1-74102] - Kv7.3 Antibody Titration: 1.0 ug/ml Positive Control: Mouse Heart.](http://images.novusbio.com/fullsize2/Kv7.3-Antibody-Western-Blot-NBP1-74102-img0003.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mAb against HEK293 (1) and CHRNA4 (AA: 29-242)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/Nicotinic-Acetylcholine-R-alpha-4-CHRNA4-Antibody-(7B4H1)-Western-Blot-NBP2-61674-img0003.jpg)
![Flow Cytometry: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mouse mAb against SH-SY5Y (1) cell lysate.](http://images.novusbio.com/fullsize2/Nicotinic Acetylcholine R alpha 4-CHRNA4 Antibody (7B4H1)-Flow Cytometry-NBP2-61674-img0004.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mAb against human CHRNA4 (AA: 29-242) recombinant protein. (Expected MW is 52.5 kDa)](http://images.novusbio.com/fullsize2/Nicotinic-Acetylcholine-R-alpha-4-CHRNA4-Antibody-(7B4H1)-Western-Blot-NBP2-61674-img0002.jpg)
![Western Blot: EFHC2 Antibody [NBP2-16279] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/EFHC2 Antibody-Western Blot-NBP2-16279-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: EFHC2 Antibody [NBP2-16279] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/EFHC2 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16279-img0003.jpg)
![Immunohistochemistry-Paraffin: EFHC2 Antibody [NBP2-16279] - Immunohistochemical analysis of paraffin-embedded U373 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/EFHC2 Antibody-Immunohistochemistry-Paraffin-NBP2-16279-img0002.jpg)
![Western Blot: Lgi1 Antibody [NBP1-77320] - HeLa cell lysate with LGI1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize2/Lgi1-Antibody-Western-Blot-NBP1-77320-img0003.jpg)
![Immunohistochemistry-Paraffin: Lgi1 Antibody [NBP1-77320] - Human brain tissue using LGI1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize2/Lgi1-Antibody-Immunohistochemistry-Paraffin-NBP1-77320-img0002.jpg)
![Immunofluorescence: Lgi1 Antibody [NBP1-77320] - Human Brain cells with LGI1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize2/Lgi1-Antibody-Immunofluorescence-NBP1-77320-img0004.jpg)
![Western Blot: NEK8 Antibody [NBP1-82539] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/NEK8-Antibody-Western-Blot-NBP1-82539-img0008.jpg)
![Immunohistochemistry-Paraffin: NEK8 Antibody [NBP1-82539] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/NEK8-Antibody-Immunohistochemistry-Paraffin-NBP1-82539-img0010.jpg)
![Western Blot: NEK8 Antibody [NBP1-82539] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize2/NEK8-Antibody-Western-Blot-NBP1-82539-img0007.jpg)
![Immunohistochemistry: RHAG Antibody [NBP2-49180] - Staining of human placenta shows erythrocyte positivity.](http://images.novusbio.com/fullsize2/RHAG Antibody-Immunohistochemistry-NBP2-49180-img0001.jpg)
![Western Blot: NHLRC1 Antibody (S85-18) [NBP2-42173] - analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of 42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 42 kDa.](http://images.novusbio.com/fullsize2/NHLRC1-Antibody-(S85-18)-Western-Blot-NBP2-42173-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NHLRC1 Antibody (S85-18) [NBP2-42173] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X.](http://images.novusbio.com/fullsize2/NHLRC1 Antibody (S85-18)-Immunocytochemistry-Immunofluorescence-NBP2-42173-img0002.jpg)
![Immunohistochemistry-Paraffin: GPR98 Antibody [NLS1729] - Anti-GPR98 / VLGR1 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize2/GPR98-Antibody-Immunohistochemistry-Paraffin-NLS1729-img0006.jpg)
![Immunohistochemistry-Paraffin: GPR98 Antibody [NLS1729] - Analysis of anti-GPR98 / VLGR1 antibody with human kidney, renal tubules.](http://images.novusbio.com/fullsize2/GPR98-Antibody-Immunohistochemistry-Paraffin-NLS1729-img0002.jpg)
![Immunohistochemistry-Paraffin: GPR98 Antibody [NLS1729] - Analysis of anti-GPR98 / VLGR1 antibody with human brain, cortex.](http://images.novusbio.com/fullsize2/GPR98-Antibody-Immunohistochemistry-Paraffin-NLS1729-img0003.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")
