![Western Blot: EFHC1 Antibody [H00114327-D01P] - Analysis of EFHC1 expression in mouse testis.](http://images.novusbio.com/fullsize2/EFHC1-Antibody-Western-Blot-H00114327-D01P-img0003.jpg "Western Blot: EFHC1 Antibody [H00114327-D01P] - Analysis of EFHC1 expression in mouse testis.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | EFHC1 (AAH20210.1, 1 a.a. ~ 640 a.a) full-length human protein.MVSNPVHGLPFLPGTSFKDSTKTAFHRSQTLSYRNGYAIVRRPTVGIGGDRLQFNQLSQAELDELASKAPVLTYGQPKQAPPADFIPAHVAFDKKVLKFDAYFQEDVPMSTEEQYRIRQVNIYYYLEDDSMSVIEPVVENSGILQGKLIKRQRLAKNDRGDHYHWKDLNRGINITIYGKTFRVVDCDQFTQVFLESQGIELNPPEKMALDPYTELRKQPLRKYVTPSDFDQLKQFLTFDKQVLRFYAIWDDTDSM |
| Specificity | Reacts with EF-hand domain (C-terminal) containing 1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EFHC1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | This antibody is reactive against tissue and transfected lysate in western blot, and as a detection antibody in ELISA. |
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | Protein A purified |
| Publication using H00114327-D01P | Applications | Species |
|---|---|---|
| Raju PK, Satishchandra P, Nayak S et al. Microtubule-associated defects caused by EFHC1 mutations in juvenile myoclonic epilepsy. Hum Mutat 2017 Mar 31 [PMID: 28370826] |
Secondary Antibodies |
Isotype Controls |
Diseases for EFHC1 Antibody (H00114327-D01P)Discover more about diseases related to EFHC1 Antibody (H00114327-D01P).
| Pathways for EFHC1 Antibody (H00114327-D01P)View related products by pathway.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | EFHC1 |
| Entrez |
|
| Uniprot |
|
![Western Blot: EFHC1 Antibody [H00114327-D01P] - Analysis of EFHC1 expression in transfected 293T cell line by EFHC1 polyclonal antibody.Lane 1: EFHC1 transfected lysate(73.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/EFHC1 Antibody-Western Blot-H00114327-D01P-img0004.jpg "Western Blot: EFHC1 Antibody [H00114327-D01P] - Analysis of EFHC1 expression in transfected 293T cell line by EFHC1 polyclonal antibody.Lane 1: EFHC1 transfected lysate(73.90 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: GABA-A R alpha 1 Antibody [NB300-191] - Mouse forebrain lysates from Wild Type (WT) and alpha 1-knockout (K/O) animals showing specific immunolabeling of the -51 kDa alpha 1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 1-knockout animals.](http://images.novusbio.com/fullsize2/GABA-A-R-alpha-1-Antibody-Western-Blot-NB300-191-img0003.jpg)
![Western Blot: BRD2 Antibody [NBP1-84310] - Analysis in human cell lines HEK293 and MCF-7 using Anti-BRD2 antibody. Corresponding BRD2 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize2/BRD2-Antibody-Western-Blot-NBP1-84310-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-84310] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.](http://images.novusbio.com/fullsize2/BRD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84310-img0004.jpg)
![Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-84310] - Staining in human testis and liver tissues. Corresponding BRD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/BRD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84310-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CLCN2 Antibody [NBP1-85585] - Staining of human cell line CACO-2 shows localization to cytosol.](http://images.novusbio.com/fullsize2/CLCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85585-img0005.jpg)
![Immunohistochemistry-Paraffin: CLCN2 Antibody [NBP1-85585] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize2/CLCN2 Antibody-Immunohistochemistry-Paraffin-NBP1-85585-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Scn1a Antibody [NBP2-58876] - Staining of human cell line ASC TERT1 shows localization to nucleoplasm & plasma membrane.](http://images.novusbio.com/fullsize2/Scn1a Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58876-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Protocadherin beta 11 Antibody [NBP2-13738] - Staining of human cell line RH-30 shows localization to plasma membrane.](http://images.novusbio.com/fullsize2/Protocadherin-beta-11-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13738-img0002.jpg)
![Immunohistochemistry-Paraffin: Protocadherin beta 11 Antibody [NBP2-13738] - Staining of human prostate shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize2/Protocadherin-beta-11-Antibody-Immunohistochemistry-Paraffin-NBP2-13738-img0001.jpg)
![Western Blot: ME2 Antibody [NBP1-89559] - Analysis in human cell line Daudi.](http://images.novusbio.com/fullsize2/ME2-Antibody-Western-Blot-NBP1-89559-img0010.jpg)
![Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining in human duodenum and liver tissues using anti-ME2 antibody. Corresponding ME2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/ME2-Antibody-Immunohistochemistry-Paraffin-NBP1-89559-img0009.jpg)
![Western Blot: ME2 Antibody [NBP1-89559] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/ME2-Antibody-Western-Blot-NBP1-89559-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CELSR1 Antibody [NBP2-55658] - Staining of human cell line A549 shows localization to plasma membrane.](http://images.novusbio.com/fullsize2/CELSR1 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55658-img0001.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Mouse whole brain lysate showing specific immunolabeling of the ~46 kDa gamma-2-subunit of GABAa -R.](http://images.novusbio.com/fullsize2/GABA-A-R-gamma-2-Antibody-Western-Blot-NB300-190-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - Immunostaining of mouse retina showing specific labeling of the GABAa gamma-2 subunit in green, calbindin in red and DNA in blue. Photo courtesy of Dr. Arlene Hirano, UCLA.](http://images.novusbio.com/fullsize2/GABA-A-R-gamma-2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-190-img0001.jpg)
![Immunohistochemistry-Paraffin: Kv7.2 Antibody [NBP1-88645] - Staining of human cerebellum shows moderate nuclear membranous positivity in Purkinje cells.](http://images.novusbio.com/fullsize2/Kv7.2-Antibody-Immunohistochemistry-Paraffin-NBP1-88645-img0001.jpg)
![Western Blot: Kv7.3 Antibody [NBP1-74102] - Lanes: 100 ug CHO cell lysate Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Goat anti-rabbit HRP Secondary Antibody Dilution: 1 : 25000 Gene name: Kcnq3 Submitted by: Anonymous.](http://images.novusbio.com/fullsize2/Kv7.3 Antibody-Western Blot-NBP1-74102-img0004.jpg)
![Western Blot: Kv7.3 Antibody [NBP1-74102] - Kv7.3 Antibody Titration: 1.0 ug/ml Positive Control: Mouse Heart.](http://images.novusbio.com/fullsize2/Kv7.3-Antibody-Western-Blot-NBP1-74102-img0003.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mAb against HEK293 (1) and CHRNA4 (AA: 29-242)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/Nicotinic-Acetylcholine-R-alpha-4-CHRNA4-Antibody-(7B4H1)-Western-Blot-NBP2-61674-img0003.jpg)
![Flow Cytometry: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mouse mAb against SH-SY5Y (1) cell lysate.](http://images.novusbio.com/fullsize2/Nicotinic Acetylcholine R alpha 4-CHRNA4 Antibody (7B4H1)-Flow Cytometry-NBP2-61674-img0004.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mAb against human CHRNA4 (AA: 29-242) recombinant protein. (Expected MW is 52.5 kDa)](http://images.novusbio.com/fullsize2/Nicotinic-Acetylcholine-R-alpha-4-CHRNA4-Antibody-(7B4H1)-Western-Blot-NBP2-61674-img0002.jpg)
![Western Blot: EFHC2 Antibody [NBP2-16279] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/EFHC2 Antibody-Western Blot-NBP2-16279-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: EFHC2 Antibody [NBP2-16279] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/EFHC2 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16279-img0003.jpg)
![Immunohistochemistry-Paraffin: EFHC2 Antibody [NBP2-16279] - Immunohistochemical analysis of paraffin-embedded U373 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/EFHC2 Antibody-Immunohistochemistry-Paraffin-NBP2-16279-img0002.jpg)
![Western Blot: Lgi1 Antibody [NBP1-77320] - HeLa cell lysate with LGI1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize2/Lgi1-Antibody-Western-Blot-NBP1-77320-img0003.jpg)
![Immunohistochemistry-Paraffin: Lgi1 Antibody [NBP1-77320] - Human brain tissue using LGI1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize2/Lgi1-Antibody-Immunohistochemistry-Paraffin-NBP1-77320-img0002.jpg)
![Immunofluorescence: Lgi1 Antibody [NBP1-77320] - Human Brain cells with LGI1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize2/Lgi1-Antibody-Immunofluorescence-NBP1-77320-img0004.jpg)
![Western Blot: NEK8 Antibody [NBP1-82539] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/NEK8-Antibody-Western-Blot-NBP1-82539-img0008.jpg)
![Immunohistochemistry-Paraffin: NEK8 Antibody [NBP1-82539] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/NEK8-Antibody-Immunohistochemistry-Paraffin-NBP1-82539-img0010.jpg)
![Western Blot: NEK8 Antibody [NBP1-82539] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize2/NEK8-Antibody-Western-Blot-NBP1-82539-img0007.jpg)
![Immunohistochemistry: RHAG Antibody [NBP2-49180] - Staining of human placenta shows erythrocyte positivity.](http://images.novusbio.com/fullsize2/RHAG Antibody-Immunohistochemistry-NBP2-49180-img0001.jpg)
![Western Blot: NHLRC1 Antibody (S85-18) [NBP2-42173] - analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of 42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 42 kDa.](http://images.novusbio.com/fullsize2/NHLRC1-Antibody-(S85-18)-Western-Blot-NBP2-42173-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NHLRC1 Antibody (S85-18) [NBP2-42173] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X.](http://images.novusbio.com/fullsize2/NHLRC1 Antibody (S85-18)-Immunocytochemistry-Immunofluorescence-NBP2-42173-img0002.jpg)
![Immunohistochemistry-Paraffin: GPR98 Antibody [NLS1729] - Anti-GPR98 / VLGR1 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize2/GPR98-Antibody-Immunohistochemistry-Paraffin-NLS1729-img0006.jpg)
![Immunohistochemistry-Paraffin: GPR98 Antibody [NLS1729] - Analysis of anti-GPR98 / VLGR1 antibody with human kidney, renal tubules.](http://images.novusbio.com/fullsize2/GPR98-Antibody-Immunohistochemistry-Paraffin-NLS1729-img0002.jpg)
![Immunohistochemistry-Paraffin: GPR98 Antibody [NLS1729] - Analysis of anti-GPR98 / VLGR1 antibody with human brain, cortex.](http://images.novusbio.com/fullsize2/GPR98-Antibody-Immunohistochemistry-Paraffin-NLS1729-img0003.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0005.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.")
