CLCN2 Antibody (3E1) - Azide and BSA Free

Western Blot: CLCN2 Antibody (3E1) [H00001181-M01] - Analysis of CLCN2 expression in transfected 293T cell line by CLCN2 monoclonal antibody (M01), clone 3E1.Lane 1: CLCN2 transfected lysate(96.8 KDa).Lane 2: Non-transfected lysate.

Western Blot: CLCN2 Antibody (3E1) [H00001181-M01] - Analysis of CLCN2 over-expressed 293 cell line, cotransfected with CLCN2 Validated Chimera RNAi ( Cat # H00001181-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CLCN2 monoclonal antibody (M01), clone 3E1 (Cat # H00001181-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: CLCN2 Antibody (3E1) [H00001181-M01] - CLCN2 monoclonal antibody (M01), clone 3E1 Analysis of CLCN2 expression in Hela S3 NE.

Sandwich ELISA: CLCN2 Antibody (3E1) [H00001181-M01] - Detection limit for recombinant GST tagged CLCN2 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Mycoplasma
• Clone: 3E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CLCN2 Antibody (3E1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CLCN2 (AAH21578.1, 38 a.a. ~ 387 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TVSTAVIVFELTGQIAHILPVMIVVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQERRATQTSPLSDQEGPPTPEASVCFQVNTEDSAFPAARGETHKPLKPALKRGPSVTRNLGESPTGSAESAGIALRSLFCGSPPPEAASEKLESCEKRKLKRVRISLASDADLEGEMSPEETHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPHSRHGLPREGSPSDSDDKCQ
• Specificity: CLCN2 - chloride channel 2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLCN2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and RNAi Validation.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CLCN2 Antibody (3E1) - Azide and BSA Free

• chloride channel 2
• chloride channel protein 2
• CIC-2
• CLC2
• clC-2
• ECA2
• ECA3
• EGI11
• EGI3
• EGMA
• EJM6
• EJM8

Background

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cellvolume; membrane potential stabilization, signal transduction and transepithelial transport

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLCN2
• Entrez:
  • 1181(Human)
• OMIM:
  • 600570(Human)
• Uniprot:
  • AAH21578(Human)