GABA-A R delta Antibody

Western Blot: GABA-A R delta Antibody [NB300-200] - mouse whole brain (1) and mouse synaptic plasma membrane (2) lysates showing specific immunolabeling of the Approx. 50 kDa GAmma-subunit of the GABAA-R.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-Fr, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GABA-A R delta Antibody Summary

• Immunogen: Fusion protein from the N-terminus of the delta subunit. Accession # P18506
• Localization: Multi-pass membrane protein
• Specificity: Specific for the ~52k delta-subunit of the GABAA receptor.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABRD
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:250
  • Immunoprecipitation 10 ul/50 ug lysate
  • Knockout Validated
  • Western Blot 1:1000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 22609410). Knockout Validated reported in scientific literature (PMID: 31209152).
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22609410).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Notes

As of 1/20/2017, this is a new lot of this antbody. It was produced by the same methods, using the same fusion protein antigen in new animals.

Alternate Names for GABA-A R delta Antibody

• EIG10
• EJM7
• GABA A R delta
• GABA(A) receptor subunit delta
• GABA-A receptor, delta polypeptide
• GABAAR delta
• GABAARd
• GABRD
• gamma-aminobutyric acid (GABA) A receptor, delta
• gamma-aminobutyric acid receptor subunit delta
• GEFSP5
• MGC45284

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four bs and four gs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for a- and b-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a g-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003). More recently there have been a number of studies demonstrating that the a-subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-A R delta Antibody (NB300-200)(13)

Publications using NB300-200

• Michalettos G, Walter HL, Antunes ARP Et al. Effect of Anti-inflammatory Treatment with AMD3100 and CX3CR1 Deficiency on GABAA Receptor Subunit and Expression of Glutamate Decarboxylase Isoforms After Stroke Molecular neurobiology 2021-08-20 [PMID: 34417725] (WB, Mouse)
• Sharma D, Dixit AB, Dey S et al. Increased levels of alpha 4-containing GABAA receptors in focal cortical dysplasia: A possible cause of benzodiazepine resistance Neurochemistry international 2021-05-27 [PMID: 34052299]
• Morrison, K E, Cole, A B Et al. Pubertal adversity alters chromatin dynamics and stress circuitry in the pregnant brain. Neuropsychopharmacology 2020-07-01 [PMID: 32045935] (WB, Human)
• Trent S, Hall J, Connelly WM, Errington AC Cyfip1 Haploinsufficiency Does Not Alter GABAA Receptor delta-Subunit Expression and Tonic Inhibition in Dentate Gyrus PV+ Interneurons and Granule Cells G3 (Bethesda). 2019-06-28 [PMID: 31209152] (Knockout Validated, WB, Mouse)
• Chen X, Li Z, Zhang B et al. Alleviation of mechanical allodynia by 14,15-EET in a central post-stroke pain model: possible role of allopregnanolone and dGABAAR J Pain 2018-11-27 [PMID: 30500366] (WB, Rat)
• Popoola DO, Cameron NM. Maternal care-related differences in males and females rats' sensitivity to ethanol and the associations between the GABAergic system and steroids in males. Dev Psychobiol. 2018-02-14 [PMID: 29442358] (WB, Rat)
• Popoola DO, Nizhnikov ME, Cameron NM. Strain-specific programming of prenatal ethanol exposure across generations. Alcohol: An International Biomedical Journal 2017-01-03 [PMID: 28433421] (WB, Rat)
• Borrow AP, Cameron NM, . Maternal care and affective behavior in female offspring: Implication of the neurosteroid/GABAergic system. Psychoneuroendocrinology 2017-02-01 [PMID: 27883962]
• Chugh D, Ali I, Bakochi A et al. Alterations in Brain Inflammation, Synaptic Proteins, and Adult Hippocampal Neurogenesis during Epileptogenesis in Mice Lacking Synapsin2. PLoS ONE 2015-07-16 [PMID: 26177381] (WB, Mouse)
• Carlson SL, O'Buckley TK, Thomas R et al. Altered GABAA Receptor Expression and Seizure Threshold Following Acute Ethanol Challenge in Mice Lacking the RIIbeta Subunit of PKA. Neurochem Res. 2013-10-09 [PMID: 24104609] (WB, Mouse)
• Shen H, Mohammad A, Ramroop J, Smith SS. A stress steroid triggers anxiety via increased expression of alpha4betadelta GABAA receptors in methamphetamine dependence. Neuroscience. 2013-08-28 [PMID: 23994152] (WB, Rat)
• Aarti Kuver, Hui Shen, Sheryl S. Smith Regulation of the surface expression of ?4?2? GABAA receptors by high efficacy states Brain Research 2021-06-29 [PMID: 22609410] (ICC/IF, Human)
• Herman MA, Gillis RA, Vicini S, Dretchen KL, Sahibzada N. Tonic GABAA receptor conductance in medial subnucleus of the tractus solitarius neurons is inhibited by activation of ?-opioid receptors. J Neurophysiol;107(3):1022-31. 2012-02-01 [PMID: 22114164] (IHC, Rat)

Reviews for GABA-A R delta Antibody (NB300-200) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 01/31/2017

Summary

• Application: Western Blot
• Sample Tested: Adolescent and adult cerebellum
• Species: Rat

reviewed by: Verified Customer WB Rat 09/26/2014

Summary

• Application: Western Blot
• Sample Tested: Rat brain synaptosomal (P2) fraction
• Species: Rat

Bioinformatics

• Gene Symbol: GABRD
• Entrez:
  • 2563(Human)
  • 14403(Mouse)
  • 29689(Rat)
• Uniprot:
  • P18506(Rat)