GABA-A R delta Antibody

Images

 
Western Blot: GABA-A R delta Antibody [NB300-200] - mouse whole brain (1) and mouse synaptic plasma membrane (2) lysates showing specific immunolabeling of the Approx. 50 kDa GAmma-subunit of the GABAA-R.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-Fr, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GABA-A R delta Antibody Summary

Immunogen
Fusion protein from the N-terminus of the delta subunit
Localization
Multi-pass membrane protein
Specificity
Specific for the ~52k delta-subunit of the GABAA receptor.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GABRD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:250
  • Immunoprecipitation 10 ul/50 ug lysate
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-200 in the following applications:

Publications
Read Publications using
NB300-200 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    5 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22609410).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

As of 1/20/2017, this is a new lot of this antbody. It was produced by the same methods, using the same fusion protein antigen in new animals.

Alternate Names for GABA-A R delta Antibody

  • EIG10
  • EJM7
  • GABA A R delta
  • GABA(A) receptor subunit delta
  • GABA-A receptor, delta polypeptide
  • GABAAR delta
  • GABAARd
  • GABRD
  • gamma-aminobutyric acid (GABA) A receptor, delta
  • gamma-aminobutyric acid receptor subunit delta
  • GEFSP5
  • MGC45284

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four bs and four gs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for a- and b-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a g-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003). More recently there have been a number of studies demonstrating that the a-subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GABA-A R delta Antibody (NB300-200)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
WB
(5)
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Filter By Species
Human
(1)
Mouse
(2)
Rat
(4)
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Showing Publications 1 - 8 of 8.
Publications using NB300-200 Applications Species
Popoola DO, Cameron NM. Maternal care-related differences in males and females rats' sensitivity to ethanol and the associations between the GABAergic system and steroids in males. Dev Psychobiol. 2018 Feb 14 [PMID: 29442358] (WB, Rat) WB Rat
Popoola DO, Nizhnikov ME, Cameron NM. Strain-specific programming of prenatal ethanol exposure across generations. Alcohol: An International Biomedical Journal 2017 Jan 03 [PMID: 28433421] (WB, Rat) WB Rat
Borrow AP, Cameron NM,. Maternal care and affective behavior in female offspring: Implication of the neurosteroid/GABAergic system. Psychoneuroendocrinology 2017 Feb [PMID: 27883962]
Chugh D, Ali I, Bakochi A et al. Alterations in Brain Inflammation, Synaptic Proteins, and Adult Hippocampal Neurogenesis during Epileptogenesis in Mice Lacking Synapsin2. PLoS ONE 2015 Jul 16 [PMID: 26177381] (WB, Mouse) WB Mouse
Carlson SL, O'Buckley TK, Thomas R et al. Altered GABAA Receptor Expression and Seizure Threshold Following Acute Ethanol Challenge in Mice Lacking the RIIbeta Subunit of PKA. Neurochem Res. 2013 Oct 9 [PMID: 24104609] (WB, Mouse) WB Mouse
Shen H, Mohammad A, Ramroop J, Smith SS. A stress steroid triggers anxiety via increased expression of alpha4betadelta GABAA receptors in methamphetamine dependence. Neuroscience. 2013 Aug 28 [PMID: 23994152] (WB, Rat) WB Rat
Aarti Kuver, Hui Shen, Sheryl S. Smith, Regulation of the surface expression of ?4?2? GABAA receptors by high efficacy states, Brain Research, 10. Brain Research, 10 1016/jbrainres.04.047. 2012 [PMID: 22609410] (ICC/IF, Human) ICC/IF Human
Herman MA, Gillis RA, Vicini S, Dretchen KL, Sahibzada N. Tonic GABAA receptor conductance in medial subnucleus of the tractus solitarius neurons is inhibited by activation of ?-opioid receptors. J Neurophysiol;107(3):1022-31. 2012 Feb. [PMID: 22114164] (IHC, Rat) IHC Rat

Reviews for GABA-A R delta Antibody (NB300-200) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Rat.

Reviews using NB300-200:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot GABA-A R delta NB300-200
Enlarge
reviewed by:
Daniel Popoola
WB Rat 01/31/2017
View

Summary

ApplicationWestern Blot
Sample TestedAdolescent and adult cerebellum
SpeciesRat
Western Blot GABA-A R delta NB300-200
Enlarge
reviewed by:
Daniel Popoola
WB Rat 09/26/2014
View

Summary

ApplicationWestern Blot
Sample TestedRat brain synaptosomal (P2) fraction
SpeciesRat
CommentsPrimary antibody dilution can be reused several times and still works great. However, strength of detection decreases over time thus creating necessity for a longer period of exposure to the film. Increasing concentration of secondary antibody to 1:7500 also appears to enhance detection strength on the film.

Blocking

Blocking Details0.1% bovine serum albumin in PBS-1% Tween 20

Primary Anitbody

Dilution RatioPrimary antibody was diluted to 1:500 in blocking buffer solution and membrane was incubated over 24 hours at 4 degrees.

Secondary Antibody

Secondary DescriptionGoat anti-rabbit
Secondary Manufacturer Cat#Thermofisher scientific
Secondary Concentration1:10000 in blocking buffer

Details

Detection NotesEnhanced chemiluminescence was used and membranes were exposed to film for 30 minutes

Comments

CommentsPrimary antibody dilution can be reused several times and still works great. However, strength of detection decreases over time thus creating necessity for a longer period of exposure to the film. Increasing concentration of secondary antibody to 1:7500 also appears to enhance detection strength on the film.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Daniel Popoola
01/31/2017
Application: WB
Species: Rat

Daniel Popoola
09/26/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol GABRD
Entrez
Uniprot