Human GIP ELISA Kit (Colorimetric)

ELISA: Human GIP ELISA Kit (Colorimetric) [NBP2-82161] - Samples were spiked with high concentrations of Human GIP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human GIP ELISA Kit (Colorimetric) [NBP2-82161] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL

Human GIP ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human GIP in samples. No Significant cross-reactivity or interference between Human GIP and analogues was observed.
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 6%
• Intra-Assay: CV% < 5%
• Spike Recovery: average = 99% for serum; average = 97% for EDTA plasma; average = 98% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GIP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human GIP ELISA Kit (Colorimetric)

• gastric inhibitory polypeptide
• GIP
• Glucose-dependent Insulinotropic Polypeptide
• Incretin

Background

The GIP gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nu

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for GIP ELISA Kit (NBP2-82161)(1)

Publication using NBP2-82161

• Figge A, Sydor S, Wenning C et al. Gender and gut microbiota composition determine hepatic bile acid, metabolic and inflammatory response to a single fast-food meal in healthy adults Clinical nutrition (Edinburgh, Scotland) 2021-04-20 [PMID: 33933727] (ELISA)

FAQs for GIP ELISA Kit (NBP2-82161). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GIP