FUS Antibody

Images

 
Genetic Strategies: Western Blot: FUS Antibody [NBP1-89113] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-FUS antibody. Remaining relative intensity ...read more
Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP1-89113] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining in human endometrium and liver tissues. Corresponding FUS RNA-seq data are presented for the same tissues.
Western Blot: FUS Antibody [NBP1-89113] - Analysis in human cell line HeLa.
Western Blot: FUS Antibody [NBP1-89113] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - FFPE 5micron sections, Citrate pretreatment, 1:500 20m, Ultravision DAB detection. This image was submitted via customer review.
Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human cerebellum shows strong nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human endometrium shows strong nuclear positivity in glandular cells and stromal cells.
Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human liver shows moderate nuclear positivity in bile duct cells while hepatocytes are negative.
Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

FUS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDR
Specificity
Specificity of human FUS antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FUS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, PFA/Triton X-100 Immunohistochemistry-Paraffin verified by a customer review.
Control
FUS Knockout 293T Cell Lysate
Control Peptide
Reviewed Applications
Read 1 Review rated 5
using
NBP1-89113 in the following applications:

Publications
Read Publications using
NBP1-89113 in the following applications:

  • 3 publications
  • IHC
    7 publications
  • WB
    2 publications

Reactivity Notes

Reactivity reported in scientific literature (PMID: 19669651)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FUS Antibody

  • 75 kDa DNA-pairing protein
  • ALS6
  • amyotrophic lateral sclerosis 6
  • FUS1
  • fused in sarcoma
  • fusion (involved in t(12;16) in malignant liposarcoma)
  • fusion gene in myxoid liposarcoma
  • fusion, derived from t(12;16) malignant liposarcoma
  • fus-like protein
  • heterogeneous nuclear ribonucleoprotein P2
  • HNRNPP2
  • Oncogene FUS
  • Oncogene TLS
  • POMP75
  • RNA-binding protein FUS
  • TLShnRNP-P2
  • Translocated in liposarcoma protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FUS Antibody (NBP1-89113)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(3)
IHC
(7)
WB
(2)
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Human
(4)
Mouse
(1)
Rat
(1)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NBP1-89113 Applications Species
Ziskin JL, Greicius MD, Zhu W et al. Neuropathologic analysis of Tyr69His TTR variant meningovascular amyloidosis with dementia. Acta Neuropathol Commun 2015 Jul 10 [PMID: 26156087] (IHC, Human) IHC Human
Barmada SJ, Ju S, Arjun A et al. Amelioration of toxicity in neuronal models of amyotrophic lateral sclerosis by hUPF1. Proc Natl Acad Sci U S A 2015 Jun 23 [PMID: 26056265] (IHC, Rat) IHC Rat
Ayaki T, Ito H, Fukushima H et al. Immunoreactivity of valosin-containing protein in sporadic amyotrophic lateral sclerosis and in a case of its novel mutant. Acta Neuropathol Commun 2014 Dec 10 [PMID: 25492614] (IHC, Human) IHC Human
Sephton CF, Tang AA, Kulkarni A et al. Activity-dependent FUS dysregulation disrupts synaptic homeostasis. Proc Natl Acad Sci U S A 2014 Nov 4 [PMID: 25324524] (IHC, WB, Mouse) IHC, WB Mouse
Nakamura M, Murray ME, Lin WL et al. Optineurin immunoreactivity in neuronal and glial intranuclear inclusions in adult-onset neuronal intranuclear inclusion disease. Am J Neurodegener Dis 2014 [PMID: 25232514] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Qiu H, Lee S, Shang Y et al. ALS-associated mutation FUS-R521C causes DNA damage and RNA splicing defects. J Clin Invest 2014 Mar [PMID: 24509083]
Dobson-Stone C, Luty AA, Thompson EM et al. Frontotemporal dementia-amyotrophic lateral sclerosis syndrome locus on chromosome 16p12.1-q12.2: genetic, clinical and neuropathological analysis. Acta Neuropathol 2013 Apr [PMID: 23338750]
Dormann D, Madl T, Valori CF et al. Arginine methylation next to the PY-NLS modulates Transportin binding and nuclear import of FUS. EMBO J 2012 Nov 14 [PMID: 22968170] (IHC, ICC/IF) IHC, ICC/IF
Snowden JS, Rollinson S, Thompson JC et al. Distinct clinical and pathological characteristics of frontotemporal dementia associated with C9ORF72 mutations. Brain 2012 Mar [PMID: 22300873]
Neumann M, Bentmann E, Dormann D et al. FET proteins TAF15 and EWS are selective markers that distinguish FTLD with FUS pathology from amyotrophic lateral sclerosis with FUS mutations. Brain 2011 Sep [PMID: 21856723]
Show All 19 Publications.

Review for FUS Antibody (NBP1-89113) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-89113:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin FUS NBP1-89113
Enlarge
reviewed by:
Kristi Bailey
IHC-P Human 01/26/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman brain
SpeciesHuman
LotJ114737

Comments

CommentsFFPE 5micron sections, Citrate pretreatment, 1:500 20m, Ultravision DAB detection

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FUS Antibody (NBP1-89113) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional FUS Products

Bioinformatics Tool for FUS Antibody (NBP1-89113)

Discover related pathways, diseases and genes to FUS Antibody (NBP1-89113). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for FUS Antibody (NBP1-89113)

Discover more about diseases related to FUS Antibody (NBP1-89113).
 

Pathways for FUS Antibody (NBP1-89113)

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PTMs for FUS Antibody (NBP1-89113)

Learn more about PTMs related to FUS Antibody (NBP1-89113).
 

Research Areas for FUS Antibody (NBP1-89113)

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Blogs on FUS.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

Amyotrophic Lateral Sclerosis Infographic
Amyotrophic lateral sclerosis is a neurological disease which impacts motor neurons that are involved in muscle movement throughout the body. The progressive degeneration of neurons causes weakened muscles and can lead to paralysis. There is no cure f...  Read full blog post.

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Recent Reviews

5
5
1
4
0
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0
2
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1
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Kristi Bailey
01/26/2018
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol FUS