XPV/DNA polymerase eta Antibody

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Western Blot: XPV/DNA polymerase eta Antibody [NB100-60424] - Detection of Human PolH on HeLa whole cell lysate using NB100-60424. PolH was also immunoprecipitated by rabbit anti-PolH antibody NB100-60423.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

XPV/DNA polymerase eta Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 690 and 713 of human polymerase (DNA directed), eta using the numbering given in entry NP_006493.1 (GeneID 5429).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-60424.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XPV/DNA polymerase eta Antibody

  • DNA polymerase eta
  • DNA Polymerase, eta
  • EC 2.7.7.7
  • POLH
  • polymerase (DNA directed), eta
  • RAD30 homolog A
  • RAD30
  • RAD30A
  • RAD30AFLJ16395
  • Xeroderma pigmentosum variant type protein
  • XPV
  • XP-V
  • XPVFLJ21978

Background

DNA polymerase eta is specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. It also plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. It targets POLI to replication foci (referenced from swissprot).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XPV/DNA polymerase eta Antibody (NB100-60424)(2)

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Bioinformatics

Gene Symbol POLH
Entrez
Uniprot