XPV/DNA polymerase eta Antibody

Western Blot: XPV/DNA polymerase eta Antibody [NB100-60424] - Detection of human PolH by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-PolH antibody NB100-60424 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60424] - Detection of human XPV/DNA polymerase eta/PolH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-PolH antibody NB100-60424 (lot 3) used for IP at 6 ug per reaction. PolH was also immunoprecipitated by a previous lot of this antibody (lot 2) and rabbit anti-PolH antibody NB100-60423. For blotting immunoprecipitated PolH, NB100-60424 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

XPV/DNA polymerase eta Antibody Summary

• Description: Novus Biologicals Rabbit XPV/DNA polymerase eta Antibody (NB100-60424) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-XPV/DNA polymerase eta Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 690 and 713 of human polymerase (DNA directed), eta using the numbering given in entry NP_006493.1 (GeneID 5429).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 -1:10000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for XPV/DNA polymerase eta Antibody

• DNA polymerase eta
• DNA Polymerase, eta
• EC 2.7.7.7
• POLH
• polymerase (DNA directed), eta
• RAD30 homolog A
• RAD30
• RAD30A
• RAD30AFLJ16395
• Xeroderma pigmentosum variant type protein
• XPV
• XP-V
• XPVFLJ21978

Background

DNA polymerase eta is specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. It also plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. It targets POLI to replication foci (referenced from swissprot).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XPV/DNA polymerase eta Antibody (NB100-60424)(4)

Publications using NB100-60424

• Castaño BA, Schorer S, Guo Y et Al. The levels of p53 govern the hierarchy of DNA damage tolerance pathway usage Nucleic Acids Res 2024-04-24 [PMID: 38321962]
• Barkley LR, Palle K, Durando M et al. c-Jun N-terminal kinase-mediated Rad18 phosphorylation facilitates Pol? recruitment to stalled replication forks. Mol Biol Cell 2012-05-01 [PMID: 22456510]
• Day TA, Palle K, Barkley LR et al. Phosphorylated Rad18 directs DNA polymerase ? to sites of stalled replication. J Cell Biol 2010-11-01 [PMID: 21098111]
• Ihle M Connections between stem cell features, EMT, DNA Replication and DNA damage response: mechanisms and therapeutic implication Thesis 2021-01-01 (WB, PLA, ICC/IF, Rabbit)

Bioinformatics

• Gene Symbol: POLH
• Entrez:
  • 5429(Human)
• Uniprot:
  • Q9UQ35(Human)