![Western Blot: DNA polymerase theta Antibody (1C11) [H00010721-M09]](http://images.novusbio.com/fullsize2/DNA-polymerase-theta-Antibody-(1C11)-Western-Blot-H00010721-M09-img0002.jpg "Western Blot: DNA polymerase theta Antibody (1C11) [H00010721-M09]")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, S-ELISA |
| Clone | 1C11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | POLQ (NP_006587, 2572 a.a. ~ 2680 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDRTGLSRKRKLQGMFCPIRGGFFILQLHDELLYE |
| Specificity | POLQ - polymerase (DNA directed), theta (1C11) |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | POLQ |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00010721-M09 | Applications | Species |
|---|---|---|
| Srinivasan G, Williamson E, Kong K et al. MiR223-3p promotes synthetic lethality in BRCA1-deficient cancers PNAS. 2019 Aug 27 |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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Enlarge |
reviewed by:
Kara Chan |
WB | Human | 08/29/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Diseases for DNA polymerase theta Antibody (H00010721-M09)Discover more about diseases related to DNA polymerase theta Antibody (H00010721-M09).
| Pathways for DNA polymerase theta Antibody (H00010721-M09)View related products by pathway.
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![ELISA: DNA polymerase theta Antibody (1C11) [H00010721-M09] - Detection limit for recombinant GST tagged POLQ is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/DNA-polymerase-theta-Antibody-(1C11)-ELISA-H00010721-M09-img0001.jpg "ELISA: DNA polymerase theta Antibody (1C11) [H00010721-M09] - Detection limit for recombinant GST tagged POLQ is approximately 1ng/ml as a capture antibody.")
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Western-Blot-NB500-106-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Immunocytochemistry-Immunofluorescence-NB500-106-img0010.jpg)
![Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Immunohistochemistry-NB500-106-img0001.jpg)
![Knockout Validated: FUS Antibody [NB100-565] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-565) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/FUS-Antibody-Knockout-Validated-NB100-565-img0020.jpg)
![Western Blot: FUS Antibody [NB100-565] - Whole cell lysate (50 ug) from HepG2, HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Affinity purified rabbit anti-FUS antibody used for WB at 0.04 ug/mL. Detection: chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/images2/FUS-Antibody-Western-Blot-NB100-565-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody [NB100-565] - FUS (NB100-565) (green), b-III Tubulin (white), DAPI (blue). FUS antibody dilution: 1:300 in PBST (0.1% Triton X-100) + 10% GS O/N 4C. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/FUS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-565-img0022.jpg)
![Western Blot: DNA Polymerase iota Antibody [NB100-175] - Western blot analysis of DNA Polymerase iota in NIH-3T3 whole cell lysate.](http://images.novusbio.com/images/DNA-Polymerase-iota-Antibody-Western-Blot-NB100-175-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Polymerase iota Antibody [NB100-175] - The DNA Polymerase Iota Antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/images2/DNA-Polymerase-iota-Antibody-Immunocytochemistry-Immunofluorescence-NB100-175-img0002.jpg)
![Western Blot: REV1 Overexpression Lysate (Adult Normal) [NBL1-15290] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for REV1.](http://images.novusbio.com/images/REV1 Overexpression Lysate (Adult Normal)-Western Blot-NBL1-15290-img0002.jpg)
![Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/images2/AICDA Antibody-Western Blot-NBP1-30955-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-30955] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/AICDA Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30955-img0002.jpg)
![Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded mouse lymph node. AICDA antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/images2/AICDA-Antibody-Immunohistochemistry-Paraffin-NBP1-30955-img0005.jpg)
![Western Blot: EXOSC3 Antibody [NBP1-91874] - Analysis in control (vector only transfected HEK293T lysate) and EXOSC3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/EXOSC3-Antibody-Western-Blot-NBP1-91874-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: EXOSC3 Antibody [NBP1-91874] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/images2/EXOSC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91874-img0010.jpg)
![Immunohistochemistry-Paraffin: EXOSC3 Antibody [NBP1-91874] - Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal and germinal center cells.](http://images.novusbio.com/images2/EXOSC3-Antibody-Immunohistochemistry-Paraffin-NBP1-91874-img0016.jpg)
![Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - POLK monoclonal antibody (M01), clone 6F2 Analysis of POLK expression in Hela S3 NE.](http://images.novusbio.com/images2/DNA-Polymerase-Kappa-Antibody-(6F2)-Western-Blot-H00051426-M01-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/DNA-Polymerase-Kappa-Antibody-(6F2)-Immunocytochemistry-Immunofluorescence-H00051426-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/DNA-Polymerase-Kappa-Antibody-(6F2)-Immunohistochemistry-Paraffin-H00051426-M01-img0011.jpg)
![Western Blot: DNA polymerase mu Antibody [NBP2-57664] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/images2/DNA polymerase mu Antibody-Western Blot-NBP2-57664-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DNA polymerase mu Antibody [NBP2-57664] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol.](http://images.novusbio.com/images2/DNA-polymerase-mu-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57664-img0001.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.Lane 1: RAD18 transfected lysate(56.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/RAD18-Antibody-(3H7)-Western-Blot-H00056852-M01-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - The antibody was used to detect RAD18 in U2os Cells. Anti-mouse 2nd antibody was used for detection (Red). DAPI was used for nuclear staining (Blue). This image was submitted via customer Review.](http://images.novusbio.com/images2/RAD18 Antibody (3H7)-Immunofluorescence-H00056852-M01-img0018.jpg)
![Immunohistochemistry-Paraffin: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/images2/RAD18-Antibody-(3H7)-Immunohistochemistry-Paraffin-H00056852-M01-img0011.jpg)
![Western Blot: DNA Polymerase beta Antibody [NBP2-38600] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/images2/DNA-Polymerase-beta-Antibody-Western-Blot-NBP2-38600-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Polymerase beta Antibody [NBP2-38600] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/images2/DNA-Polymerase-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38600-img0004.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP2-38600] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/images2/DNA-Polymerase-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-38600-img0005.jpg)
![Western Blot: XPA Antibody [NB100-93321] - Detection of human XPA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPA antibody NB100-93321 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/images2/XPA-Antibody-Western-Blot-NB100-93321-img0005.jpg)
![Immunohistochemistry: XPA Antibody [NB100-93321] - Detection of human XPA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XPA (NB100-93321). Detection: DAB.](http://images.novusbio.com/images2/XPA Antibody-Immunohistochemistry-NB100-93321-img0006.jpg)
![Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-93321 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate](http://images.novusbio.com/images2/XPA-Antibody-Western-Blot-NB100-93321-img0002.jpg)
![Western Blot: HOMER2 Antibody [H00009455-D01P] - Analysis of HOMER2 expression in mouse brain.](http://images.novusbio.com/images2/HOMER2-Antibody-Western-Blot-H00009455-D01P-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HOMER2 Antibody [H00009455-D01P] - Analysis of antibody to HOMER2 on HeLa cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/images2/HOMER2-Antibody-Immunocytochemistry-Immunofluorescence-H00009455-D01P-img0004.jpg)
![Western Blot: HOMER2 Antibody [H00009455-D01P] - Analysis of HOMER2 expression in A-431.](http://images.novusbio.com/images2/HOMER2-Antibody-Western-Blot-H00009455-D01P-img0005.jpg)
![Western Blot: UNG Antibody [NB600-1031] - Total protein from human HeLa cells, muse Neuro2A cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-UNG in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/UNG-Antibody-Western-Blot-NB600-1031-img0003.jpg)
![Immunohistochemistry-Paraffin: uracil-DNA glycosylase Antibody [NB600-1031] - Analysis of human testis using this antibody at 10 ug/ml.](http://images.novusbio.com/images2/uracil-DNA glycosylase Antibody-Immunohistochemistry-Paraffin-NB600-1031-img0002.jpg)
![Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/images2/Rad51-Antibody-(14B4)-Western-Blot-NB100-148-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Staining of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.](http://images.novusbio.com/images2/Rad51-Antibody-(14B4)-Immunocytochemistry-Immunofluorescence-NB100-148-img0004.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - BT483 xenograft. RAD51 antibody 14B4 dilution at 1:200. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15 min.](http://images.novusbio.com/images2/Rad51-Antibody-(14B4)-Immunohistochemistry-Paraffin-NB100-148-img0003.jpg)
