![Western Blot: DNA polymerase theta Antibody (1C11) [H00010721-M09]](http://images.novusbio.com/fullsize2/DNA-polymerase-theta-Antibody-(1C11)-Western-Blot-H00010721-M09-img0002.jpg "Western Blot: DNA polymerase theta Antibody (1C11) [H00010721-M09]")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 1C11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | POLQ (NP_006587, 2572 a.a. ~ 2680 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 DGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDRTGLSRKRKLQGMFCPIRGGFFILQLHDELLYE |
| Specificity | POLQ - polymerase (DNA directed), theta (1C11) |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | POLQ |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00010721-M09 | Applications | Species |
|---|---|---|
| Srinivasan G, Williamson E, Kong K et al. MiR223-3p promotes synthetic lethality in BRCA1-deficient cancers PNAS. 2019 Aug 27 |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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Enlarge |
reviewed by:
Kara Chan |
WB | Human | 08/29/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Diseases for DNA polymerase theta Antibody (H00010721-M09)Discover more about diseases related to DNA polymerase theta Antibody (H00010721-M09).
| Pathways for DNA polymerase theta Antibody (H00010721-M09)View related products by pathway.
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![ELISA: DNA polymerase theta Antibody (1C11) [H00010721-M09] - Detection limit for recombinant GST tagged POLQ is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/DNA-polymerase-theta-Antibody-(1C11)-ELISA-H00010721-M09-img0001.jpg "ELISA: DNA polymerase theta Antibody (1C11) [H00010721-M09] - Detection limit for recombinant GST tagged POLQ is approximately 1ng/ml as a capture antibody.")
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/PCNA-Antibody-(PC10)-Western-Blot-NB500-106-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/PCNA-Antibody-(PC10)-Immunocytochemistry-Immunofluorescence-NB500-106-img0010.jpg)
![Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.](http://images.novusbio.com/fullsize2/PCNA-Antibody-(PC10)-Immunohistochemistry-NB500-106-img0001.jpg)
![Western Blot: FUS Antibody [NBP1-89113] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-FUS antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize2/FUS-Antibody-Western-Blot-NBP1-89113-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody [NBP1-89113] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/FUS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89113-img0028.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining in human endometrium and liver tissues. Corresponding FUS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0027.jpg)
![Western Blot: DNA Polymerase iota Antibody [NB100-175] - Western blot analysis of DNA Polymerase iota in NIH-3T3 whole cell lysate.](http://images.novusbio.com/fullsize/DNA-Polymerase-iota-Antibody-Western-Blot-NB100-175-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Polymerase iota Antibody [NB100-175] - The DNA Polymerase Iota Antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize2/DNA-Polymerase-iota-Antibody-Immunocytochemistry-Immunofluorescence-NB100-175-img0002.jpg)
![Western Blot: REV1 Overexpression Lysate (Adult Normal) [NBL1-15290] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for REV1.](http://images.novusbio.com/fullsize/REV1 Overexpression Lysate (Adult Normal)-Western Blot-NBL1-15290-img0002.jpg)
![Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/AICDA Antibody-Western Blot-NBP1-30955-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-30955] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/AICDA Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30955-img0002.jpg)
![Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded mouse lymph node. AICDA antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/fullsize2/AICDA-Antibody-Immunohistochemistry-Paraffin-NBP1-30955-img0005.jpg)
![Western Blot: DNA polymerase sigma Antibody [NBP2-13728] - Analysis in human cell line HeLa.](http://images.novusbio.com/fullsize2/DNA-polymerase-sigma-Antibody-Western-Blot-NBP2-13728-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: DNA polymerase sigma Antibody [NBP2-13728] - Staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/DNA-polymerase-sigma-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13728-img0008.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase sigma Antibody [NBP2-13728] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize2/DNA-polymerase-sigma-Antibody-Immunohistochemistry-Paraffin-NBP2-13728-img0013.jpg)
![Western Blot: EXOSC3 Antibody [NBP1-91874] - Analysis in control (vector only transfected HEK293T lysate) and EXOSC3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/EXOSC3-Antibody-Western-Blot-NBP1-91874-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: EXOSC3 Antibody [NBP1-91874] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize2/EXOSC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91874-img0010.jpg)
![Immunohistochemistry: EXOSC3 Antibody [NBP1-91874] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize2/EXOSC3-Antibody-Immunohistochemistry-NBP1-91874-img0015.jpg)
![Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - POLK monoclonal antibody (M01), clone 6F2 Analysis of POLK expression in Hela S3 NE.](http://images.novusbio.com/fullsize2/DNA-Polymerase-Kappa-Antibody-(6F2)-Western-Blot-H00051426-M01-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/DNA-Polymerase-Kappa-Antibody-(6F2)-Immunocytochemistry-Immunofluorescence-H00051426-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize2/DNA-Polymerase-Kappa-Antibody-(6F2)-Immunohistochemistry-Paraffin-H00051426-M01-img0011.jpg)
![Western Blot: DNA polymerase mu Antibody [NBP2-57664] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/fullsize2/DNA polymerase mu Antibody-Western Blot-NBP2-57664-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DNA polymerase mu Antibody [NBP2-57664] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol.](http://images.novusbio.com/fullsize2/DNA-polymerase-mu-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57664-img0001.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.Lane 1: RAD18 transfected lysate(56.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/RAD18-Antibody-(3H7)-Western-Blot-H00056852-M01-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - The antibody was used to detect RAD18 in U2os Cells. Anti-mouse 2nd antibody was used for detection (Red). DAPI was used for nuclear staining (Blue). This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/RAD18 Antibody (3H7)-Immunofluorescence-H00056852-M01-img0018.jpg)
![Immunohistochemistry-Paraffin: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize2/RAD18-Antibody-(3H7)-Immunohistochemistry-Paraffin-H00056852-M01-img0011.jpg)
![Western Blot: DNA Polymerase beta Antibody (JM93-12) [NBP2-67036] - Analysis of DNA Polymerase beta on different cell lysates using anti-DNA Polymerase beta antibody at 1/1,000 dilution. Positive control:Lane 1: PC-12 Lane 2: NIH-3T3 Lane 3: A431](http://images.novusbio.com/fullsize2/DNA Polymerase beta Antibody (JM93-12)-Western Blot-NBP2-67036-img0005.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody (JM93-12) [NBP2-67036] - Analysis of paraffin-embedded rat lung tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/DNA-Polymerase-beta-Antibody-(JM93-12)-Immunohistochemistry-Paraffin-NBP2-67036-img0004.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody (JM93-12) [NBP2-67036] - Analysis of paraffin-embedded human breast cancer tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/DNA-Polymerase-beta-Antibody-(JM93-12)-Immunohistochemistry-Paraffin-NBP2-67036-img0001.jpg)
![Western Blot: XPA Antibody [NB100-93321] - Detection of human XPA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPA antibody NB100-93321 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize2/XPA-Antibody-Western-Blot-NB100-93321-img0005.jpg)
![Immunohistochemistry: XPA Antibody [NB100-93321] - Detection of human XPA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XPA (NB100-93321). Detection: DAB.](http://images.novusbio.com/fullsize2/XPA Antibody-Immunohistochemistry-NB100-93321-img0006.jpg)
![Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-93321 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate](http://images.novusbio.com/fullsize2/XPA-Antibody-Western-Blot-NB100-93321-img0002.jpg)
![Western Blot: HOMER2 Antibody [H00009455-D01P] - Analysis of HOMER2 expression in mouse brain.](http://images.novusbio.com/fullsize2/HOMER2-Antibody-Western-Blot-H00009455-D01P-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HOMER2 Antibody [H00009455-D01P] - Analysis of antibody to HOMER2 on HeLa cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/fullsize2/HOMER2-Antibody-Immunocytochemistry-Immunofluorescence-H00009455-D01P-img0004.jpg)
![Western Blot: HOMER2 Antibody [H00009455-D01P] - Analysis of HOMER2 expression in A-431.](http://images.novusbio.com/fullsize2/HOMER2-Antibody-Western-Blot-H00009455-D01P-img0005.jpg)
![Western Blot: UNG Antibody [NB600-1031] - Total protein from human HeLa cells, muse Neuro2A cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-UNG in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/UNG-Antibody-Western-Blot-NB600-1031-img0003.jpg)
![Immunohistochemistry-Paraffin: uracil-DNA glycosylase Antibody [NB600-1031] - Analysis of human testis using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize2/uracil-DNA glycosylase Antibody-Immunohistochemistry-Paraffin-NB600-1031-img0002.jpg)
![Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (70 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Rad51-Antibody-(14B4)-Western-Blot-NB100-148-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Staining of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.](http://images.novusbio.com/fullsize2/Rad51-Antibody-(14B4)-Immunocytochemistry-Immunofluorescence-NB100-148-img0004.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - BT483 xenograft. RAD51 antibody 14B4 dilution at 1:200. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15 min.](http://images.novusbio.com/fullsize2/Rad51-Antibody-(14B4)-Immunohistochemistry-Paraffin-NB100-148-img0003.jpg)
