DNA polymerase theta Antibody (1C11)

Western Blot: DNA polymerase theta Antibody (1C11) [H00010721-M09]

ELISA: DNA polymerase theta Antibody (1C11) [H00010721-M09] - Detection limit for recombinant GST tagged POLQ is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DNA polymerase theta Antibody (1C11) Summary

• Immunogen: POLQ (NP_006587, 2572 a.a. ~ 2680 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDRTGLSRKRKLQGMFCPIRGGFFILQLHDELLYE
• Specificity: POLQ - polymerase (DNA directed), theta (1C11)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLQ
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:1000
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNA polymerase theta Antibody (1C11)

• DKFZp781A0112
• DNA polymerase eta
• EC 2.7.7.7
• POLHDNA polymerase theta
• polymerase (DNA directed), theta
• PRO0327

Background

DNA polymerase theta, also referred to as POLQ, is highly expressed in the testes. POLQ has a 2,590 amino acid long isoform that is approximately 290kDa and a shorter 1,786 amino acid long isoform that is approximately 198kDa. POLQ is highly expressed in the testes, plays a role in DNA polymerase activity, and may be implicated in interstrand crosslink repair (ICR). Research on DNA polymerase theta has shown a connection to several diseases and disorders including stomach cancer, malaria, breast cancer, tuberculosis, and meningioma. POLQ has also been shown to have interactions with AP2S1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA polymerase theta Antibody (H00010721-M09)(2)

Publications using H00010721-M09

• Yasuhara T, Kato R, Yamauchi M et al. RAP80 suppresses the vulnerability of R-loops during DNA double-strand break repair Cell reports 2022-02-01 [PMID: 35108530]
• Srinivasan G, Williamson E, Kong K et al. MiR223-3p promotes synthetic lethality in BRCA1-deficient cancers Proc Natl Acad Sci U S A 2019-08-10 [PMID: 31395736]

Review for DNA polymerase theta Antibody (H00010721-M09) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Kara Chan WB Human 08/29/2017

Summary

• Application: Western Blot
• Sample Tested: cell line, whole cell lysate,Hela whole cell lysate,Purified protein,Whole HeLa and nuclear extract
• Species: Human
• Lot: f8201-1c11

Comments

• Comments: 1:1000

Bioinformatics

• Gene Symbol: POLQ
• Entrez:
  • 10721(Human)