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DNA polymerase theta Antibody (1C11)

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Western Blot: DNA polymerase theta Antibody (1C11) [H00010721-M09]
Western Blot: DNA polymerase theta Antibody (1C11) [H00010721-M09]
ELISA: DNA polymerase theta Antibody (1C11) [H00010721-M09] - Detection limit for recombinant GST tagged POLQ is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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DNA polymerase theta Antibody (1C11) Summary

Immunogen
POLQ (NP_006587, 2572 a.a. ~ 2680 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDRTGLSRKRKLQGMFCPIRGGFFILQLHDELLYE
Specificity
POLQ - polymerase (DNA directed), theta (1C11)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POLQ
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:1000
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Reviewed Applications
Read 1 Review rated 5
using
H00010721-M09 in the following applications:
Publications
Read Publications using H00010721-M09.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNA polymerase theta Antibody (1C11)

  • DKFZp781A0112
  • DNA polymerase eta
  • EC 2.7.7.7
  • POLHDNA polymerase theta
  • polymerase (DNA directed), theta
  • PRO0327

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Flow Cytometry: PCNA Antibody (PC10) [NB500-106] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.
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Immunohistochemistry-Paraffin: FUS Antibody [NB100-565] - FFPE section of mouse renal cells carcinoma. Affinity purified rabbit anti-FUS used at a dilution of 1:1,000 (1 ug/mL). Detection: DA
Western Blot: FUS Antibody [NB100-565] - Whole cell lysate (50 ug) from HepG2, HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Affinity purified rabbit anti-FUS antibody used for WB at 0.04 ug/mL. Detection: chemiluminescence with an exposure time of 10 seconds.
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Western Blot: DNA Polymerase iota Antibody [NB100-175] - Western blot analysis of DNA Polymerase iota in NIH-3T3 whole cell lysate.
Immunocytochemistry/Immunofluorescence: DNA Polymerase iota Antibody [NB100-175] - The DNA Polymerase Iota Antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).
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Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.
Species: Hu, Mu
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S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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(4 Publications)
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Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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DNA polymerase
theta Antibody (1C11)
H00010721-M09
DNA polymerase theta Antibody (1C11)
Species: Hu
Applications: WB, ELISA

Publications for DNA polymerase theta Antibody (H00010721-M09)(2)


Showing Publications 1 - 2 of 2.
Publications using H00010721-M09 Applications Species
Srinivasan G, Williamson E, Kong K et al. MiR223-3p promotes synthetic lethality in BRCA1-deficient cancers PNAS. 2019 Aug 27
Yasuhara T, Kato R, Yamauchi M et al. RAP80 suppresses the vulnerability of R-loops during DNA double-strand break repair Cell reports Feb 1 2022 [PMID: 35108530]

Review for DNA polymerase theta Antibody (H00010721-M09) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00010721-M09:
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Western Blot DNA polymerase theta H00010721-M09
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reviewed by:
Verified Customer
 Western Blot Human 08/29/2017
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Summary

ApplicationWestern Blot
Sample Testedcell line, whole cell lysate,Hela whole cell lysate,Purified protein,Whole HeLa and nuclear extract
SpeciesHuman
Lotf8201-1c11

Comments

Comments1:1000

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Western Blot DNA polymerase theta H00010721-M09
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 Verified Customer
08/29/2017
Application: Western Blot
Species: Human
read this review

Bioinformatics

Gene Symbol POLQ
Entrez