TAF15 Antibody

Images

 
Western Blot: TAF15 Antibody [NB100-567] - ALS proteins associate with the RNAP II/U1 snRNP machinery in an RNA-independent manner. IPs were carried out from RNase A-treated or untreated nuclear extract using an RNAP II ...read more
Immunohistochemistry-Paraffin: TAF15 Antibody [NB100-567] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- TAFII68 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: TAF15 Antibody [NB100-567] - Detection of human TAFII68 on HeLa whole cell lysate using NB100-567. TAFII68 was IPed with rabbit anti-TAFII68 antibodies NB100-566, NB100-567, NB100-568, and another ...read more
ALS proteins associate with the RNAP II/U1 snRNP machinery in an RNA-independent manner. IPs were carried out from RNase A-treated or untreated nuclear extract using an RNAP II or a negative control antibody (EIF4A3) ...read more
Autophagic clearance of aberrantly accumulated cytoplasmic FUS restores protein homeostasis and ameliorates survival of SL P525L iPSC-derived neurons. c) WES capillary electrophoresis and d corresponding quantification ...read more
The cytoplasmic mislocalization induced by P525L causes reduced FUS binding to several ALS-associated RBPs, promoting aggregation. a, b) Western blot analysis of FUS protein interactors in a LL and b SL neurons after ...read more
FET proteins and MATR3 associate with U1 snRNP. (b) IPs were carried out from nuclear extract using a negative control antibody (EIF4A3) or an antibody to the SNRPC subunit of the U1 snRNP followed by Westerns with the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TAF15 Antibody Summary

Immunogen
A synthetic peptide which maps to a region between residues 200 and 250 of human TAFII68 using the numbering given in Swiss-Prot entry Q92804 (GeneID 8148).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAF15
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 1-4 ug/mg lysate
  • Western Blot 1:5000-1:20000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publications using
NB100-567 in the following applications:

  • IP
    1 publication
  • KD
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TAF15 Antibody

  • 68kDa
  • hTAFII68
  • Npl3
  • RBP56TAFII68
  • RNA-binding protein 56
  • TAF(II)68
  • TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor
  • TAF2NRBP56/CSMF fusion
  • TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56)
  • TATA box-binding protein-associated factor 2N (RNA-binding protein 56)
  • TATA-binding protein-associated factor 2N
  • TBP-associated factor 15,68 kDa TATA-binding protein-associated factor

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAF15 Antibody (NB100-567)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TAF15 Antibody (NB100-567). (Showing 1 - 1 of 1 FAQs).

  1. What is the difference between NB100-566 and NB100-567?
    • Slightly different immunogen sequences regions were used for developing these TAF15 antibodies, NB100-566 and NB100-567.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TAF15
Entrez
Uniprot