FUS Antibody

Images

 
Genetic Strategies: Knockout Validated: FUS Antibody [NB100-562] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. ...read more
Independent Antibodies: Western Blot: FUS Antibody [NB100-562] - Detection of human FUS on HeLa whole cell lysate using NB100-562. For IP in B, rabbit anti-FUS antibodies NB100-560, NB100-561, NB100-562 and ...read more
Immunohistochemistry-Paraffin: FUS Antibody [NB100-562] - Section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:5,000 (0.2ug/ml) and ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, IHC, IHC-P, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

FUS Antibody Summary

Immunogen
A synthetic peptide which maps to a region between residues 500 and the C-terminus (residue 526) of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521).
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FUS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:50000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockout Validated
Application Notes
Prepare working dilution immediately before use. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
FUS Overexpression Lysate
HeLa Whole Cell Lysate
FUS Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-562 in the following applications:

Publications
Read Publications using
NB100-562 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FUS Antibody

  • 75 kDa DNA-pairing protein
  • ALS6
  • amyotrophic lateral sclerosis 6
  • FUS1
  • fused in sarcoma
  • fusion (involved in t(12;16) in malignant liposarcoma)
  • fusion gene in myxoid liposarcoma
  • fusion, derived from t(12;16) malignant liposarcoma
  • fus-like protein
  • heterogeneous nuclear ribonucleoprotein P2
  • HNRNPP2
  • Oncogene FUS
  • Oncogene TLS
  • POMP75
  • RNA-binding protein FUS
  • TLShnRNP-P2
  • Translocated in liposarcoma protein

Background

FUS is a nucleic acid-binding protein whose N-terminal half functions as a transcriptional activator domain in fusion oncoproteins found in human leukemias and liposarcomas. FUS has been reported to be associated with the nuclear matrix and has been implicated in transcription, pre-mRNA splicing processes, spliceosome assembly, and promoting undifferentiated endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FUS Antibody (NB100-562)(4)

Review for FUS Antibody (NB100-562) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-562:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot FUS NB100-562
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reviewed by:
Anonymous
WB Human 09/13/2015
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
LotA1

Blocking

Blocking Details5% milk in TBS; 1h at room temperatuere

Primary Anitbody

Dilution Ratio1:3000; 2h at room temperature

Secondary Antibody

Secondary DescriptionGoat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, Dylight 650 conjugate
Secondary Manufacturer Cat#SA5-10034
Secondary Concentration1:5000

Details

Detection NotesBioRad GelDoc XR System

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FUS Antibody (NB100-562) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional FUS Products

Bioinformatics Tool for FUS Antibody (NB100-562)

Discover related pathways, diseases and genes to FUS Antibody (NB100-562). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FUS Antibody (NB100-562)

Discover more about diseases related to FUS Antibody (NB100-562).
 

Pathways for FUS Antibody (NB100-562)

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PTMs for FUS Antibody (NB100-562)

Learn more about PTMs related to FUS Antibody (NB100-562).

Blogs on FUS.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

Amyotrophic Lateral Sclerosis Infographic
Amyotrophic lateral sclerosis is a neurological disease which impacts motor neurons that are involved in muscle movement throughout the body. The progressive degeneration of neurons causes weakened muscles and can lead to paralysis. There is no cure f...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
09/13/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol FUS
Entrez
Uniprot