FUS Antibody

Images

 
Biological Strategies: Western Blot: FUS Antibody [NB100-561] - Detection of Human and Mouse FUS by Western Blot. Samples: Whole cell lysate (50 ug) from HepG2, HeLa, 293T, and mouse NIH3T3 cells prepared using ...read more
Immunohistochemistry: FUS Antibody [NB100-561] - Detection of human FUS by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS (NB100-561). Detection: DAB
Independent Antibodies: Western Blot: FUS Antibody [NB100-561] - Detection of human FUS on HeLa whole cell lysate using NB100-561. For IP in B, rabbit anti-FUS antibodies NB100-560, NB100-561, and NB100-565 were ...read more
Immunohistochemistry-Paraffin: FUS Antibody [NB100-561] - Human prostate carcinoma. Antibody used at a dilution of 1:1,000.
Immunohistochemistry: FUS Antibody [NB100-561] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry: FUS Antibody [NB100-561] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: FUS Antibody [NB100-561] - FUS immunoprecipitation in human fibroblast cell lysates. Image from verified customer review.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FUS Antibody Summary

Immunogen
A synthetic peptide which maps to a region between residues 400 and 450 of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FUS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:50000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
FUS Overexpression Lysate
HeLa Whole Cell Lysate
FUS Knockout 293T Cell Lysate
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-561 in the following applications:

Publications
Read Publications using
NB100-561 in the following applications:

  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FUS Antibody

  • 75 kDa DNA-pairing protein
  • ALS6
  • amyotrophic lateral sclerosis 6
  • FUS1
  • fused in sarcoma
  • fusion (involved in t(12;16) in malignant liposarcoma)
  • fusion gene in myxoid liposarcoma
  • fusion, derived from t(12;16) malignant liposarcoma
  • fus-like protein
  • heterogeneous nuclear ribonucleoprotein P2
  • HNRNPP2
  • Oncogene FUS
  • Oncogene TLS
  • POMP75
  • RNA-binding protein FUS
  • TLShnRNP-P2
  • Translocated in liposarcoma protein

Background

FUS is a nucleic acid-binding protein whose N-terminal half functions as a transcriptional activator domain in fusion oncoproteins found in human leukemias and liposarcomas. FUS has been reported to be associated with the nuclear matrix and has been implicated in transcription, pre-mRNA splicing processes, spliceosome assembly, and promoting undifferentiated endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FUS Antibody (NB100-561)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IP.


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IP
(1)
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Human
(1)
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Reviews for FUS Antibody (NB100-561) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB100-561:
Filter by Applications
ICC
(1)
IP
(1)
WB
(1)
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Human
(3)
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Images Ratings Applications Species Date Details
Immunocytochemistry FUS NB100-561
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reviewed by:
Anonymous
ICC Human 11/18/2017
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Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
LotA2

Comments

CommentsBlocked 1H 25C in 1X PBS/5% normal serum/0.3% Triton™ X-100.
Antibody diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.
Immunoprecipitation FUS NB100-561
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reviewed by:
Anonymous
IP Human 11/26/2014
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Summary

ApplicationImmunoprecipitation
Sample TestedHuman fibroblasts, whole cell lysate
SpeciesHuman
LotA2

Blocking

Blocking Details5% Milk-TBS/T 1 Hour, Room Temp.

Primary Anitbody

Dilution Ratio1:5000 in 5% Milk-TBS/T overnight at 4C

Secondary Antibody

Secondary DescriptionECL Rabbit IgG, HRP-linked whole Ab (from donkey), 1mL
Secondary Manufacturer Cat#NA934-1ML
Secondary Concentration1:5000

Details

Detection NotesECL, 1 minute exposure
Immunoprecipitation DetailsRoche Agarose A beads 50ul slurry, 2 hour 4C incubation (post pre-clear), 4 washes in 1X Ripa buffer, 8 min each
Elution Description1X LDS buffer, Room temperature, 4 min, no reducing reagent.
Western Blot FUS NB100-561
Enlarge
reviewed by:
Jennifer Fox
WB Human 10/17/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman fibroblasts, whole cell lysate
SpeciesHuman
LotA2
CommentsA longer exposure had too much background and several bands. Would dilute the primary antibody out to 1:10,000. 15ug of whole cell lysate loaded, could also drop that to 5-10ug.

Blocking

Blocking Details5% Milk-TBS/T 1 Hour, Room Temp.

Primary Anitbody

Dilution Ratio1:5000 in 5% Milk-TBS/T overnight at 4C

Secondary Antibody

Secondary DescriptionECL Rabbit IgG, HRP-linked whole Ab (from donkey), 1mL
Secondary Manufacturer Cat#NA934-1ML
Secondary Concentration1:5000

Details

Detection NotesECL, touch exposure

Comments

CommentsA longer exposure had too much background and several bands. Would dilute the primary antibody out to 1:10,000. 15ug of whole cell lysate loaded, could also drop that to 5-10ug.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FUS Antibody (NB100-561) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional FUS Products

Bioinformatics Tool for FUS Antibody (NB100-561)

Discover related pathways, diseases and genes to FUS Antibody (NB100-561). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FUS Antibody (NB100-561)

Discover more about diseases related to FUS Antibody (NB100-561).
 

Pathways for FUS Antibody (NB100-561)

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PTMs for FUS Antibody (NB100-561)

Learn more about PTMs related to FUS Antibody (NB100-561).

Blogs on FUS.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

Amyotrophic Lateral Sclerosis Infographic
Amyotrophic lateral sclerosis is a neurological disease which impacts motor neurons that are involved in muscle movement throughout the body. The progressive degeneration of neurons causes weakened muscles and can lead to paralysis. There is no cure f...  Read full blog post.

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Recent Reviews

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5
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1
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Anonymous
11/18/2017
Application: ICC
Species: Human

Anonymous
11/26/2014
Application: IP
Species: Human

Jennifer Fox
10/17/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol FUS
Entrez
Uniprot