Fucosyltransferase 8/FUT8 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Fucosyltransferase 8/FUT8 Antibody [Biotin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 8/FUT8
  Asp32-Lys575
  Accession # Q9BYC5
• Specificity: Detects human Fucosyltransferase 8/FUT8 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) FUT3, rhFUT5, and rhFUT11 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FUT8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fucosyltransferase 8/FUT8 Antibody [Biotin]

• alpha 1,6 Fucosyltransferase
• alpha-(1,6)-fucosyltransferase
• alpha1-6FucT
• EC 2.4.1.68
• fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
• Fucosyltransferase 8
• FUT8
• GDP-fucose--glycoprotein fucosyltransferase
• GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
• Glycoprotein 6-alpha-L-fucosyltransferase
• MGC26465

Background

FUT8 (Fucosyltransferase 8; also alpha 1,6 Fucosyltransferase) is a 60-65 kDa member of the glycosyltransferase 23 family of enzymes. It catalyzes the addition of fucose to Asn-linked GlcNAc via an alpha 1,6 linkage. FUT8 is widely expressed, and the effects of its fucosylation vary. A lack of fucosylation on IgG potentiates ADCC activity, while an absence of fucose on the EGF R and alpha 3 beta 1 integrin decreases their activity. Human FUT8 is a type II transmembrane protein 575 amino acids (aa) in length. It contains a short cytoplasmic region (aa 1-9) plus an extended luminal domain (aa 31-575) that possesses one SH3 homology domain (aa 502-563). There are at least four potential splice variants. There is an alternate start site at Met479, a 14 aa substitution for aa 1-420, a 32 aa substitution for aa 1-161, and a two aa substitution for aa 444-575. Over aa 32-575, human FUT8 shares 97% aa identity with mouse FUT8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FUT8
• Uniprot:
  • Q9BYC5()