Fucosyltransferase 8/FUT8 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Fucosyltransferase 8/FUT8 Antibody [Biotin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 8/FUT8
  Asp32-Lys575
  Accession # Q9BYC5
• Specificity: Detects human Fucosyltransferase 8/FUT8 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) FUT3, rhFUT5, and rhFUT11 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FUT8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fucosyltransferase 8/FUT8 Antibody [Biotin]

• alpha 1,6 Fucosyltransferase
• alpha-(1,6)-fucosyltransferase
• alpha1-6FucT
• EC 2.4.1.68
• fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
• Fucosyltransferase 8
• FUT8
• GDP-fucose--glycoprotein fucosyltransferase
• GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
• Glycoprotein 6-alpha-L-fucosyltransferase
• MGC26465

Background

FUT8 (Fucosyltransferase 8; also alpha 1,6 Fucosyltransferase) is a 60‑65 kDa member of the glycosyltransferase 23 family of enzymes. It catalyzes the addition of fucose to Asn-linked GlcNAc via an alpha 1,6 linkage. FUT8 is widely expressed, and the effects of its fucosylation vary. A lack of fucosylation on IgG potentiates ADCC activity, while an absence of fucose on the EGF R and alpha 3 beta 1 integrin decreases their activity. Human FUT8 is a type II transmembrane protein 575 amino acids (aa) in length. It contains a short cytoplasmic region (aa 1‑9) plus an extended luminal domain (aa 31‑575) that possesses one SH3 homology domain (aa 502‑563). There are at least four potential splice variants. There is an alternate start site at Met479, a 14 aa substitution for aa 1‑420, a 32 aa substitution for aa 1‑161, and a two aa substitution for aa 444‑575. Over aa 32‑575, human FUT8 shares 97% aa identity with mouse FUT8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FUT8
• Uniprot:
  • Q9BYC5()