Fucosyltransferase 8/FUT8 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Fucosyltransferase 8/FUT8 Antibody [Alexa Fluor® 350] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 8/FUT8
  Asp32-Lys575
  Accession # Q9BYC5
• Specificity: Detects human Fucosyltransferase 8/FUT8 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fucosyltransferase 8/FUT8 Antibody [Alexa Fluor® 350]

• alpha 1,6 Fucosyltransferase
• alpha-(1,6)-fucosyltransferase
• alpha1-6FucT
• EC 2.4.1.68
• fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
• Fucosyltransferase 8
• FUT8
• GDP-fucose--glycoprotein fucosyltransferase
• GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
• Glycoprotein 6-alpha-L-fucosyltransferase
• MGC26465

Background

FUT8 (Fucosyltransferase 8; also alpha 1,6 Fucosyltransferase) is a 60-65 kDa member of the glycosyltransferase 23 family of enzymes. It catalyzes the addition of fucose to Asn-linked GlcNAc via an alpha 1,6 linkage. FUT8 is widely expressed, and the effects of its fucosylation vary. A lack of fucosylation on IgG potentiates ADCC activity, while an absence of fucose on the EGF R and alpha 3 beta 1 integrin decreases their activity. Human FUT8 is a type II transmembrane protein 575 amino acids (aa) in length. It contains a short cytoplasmic region (aa 1-9) plus an extended luminal domain (aa 31-575) that possesses one SH3 homology domain (aa 502-563). There are at least four potential splice variants. There is an alternate start site at Met479, a 14 aa substitution for aa 1-420, a 32 aa substitution for aa 1-161, and a two aa substitution for aa 444-575. Over aa 32-575, human FUT8 shares 97% aa identity with mouse FUT8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9BYC5()