Recombinant Human N-Acetylglucosaminyltransferase 3/MGAT3 CF


There are currently no images for N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Human N-Acetylglucosaminyltransferase 3/MGAT3 CF Summary

Details of Functionality
Measured by its ability to transfer N-Acetyl-alpha -D-glucosamine from UDP-N-Acetyl-alpha -D-glucosamine to a biantennary N-linked core pentasaccharide in a CD39L3 coupled assay. The specific activity is >100 pmol/min/μg, as measured under the described conditions.
Chinese Hamster Ovary cell line, CHO-derived human N-Acetylglucosaminyltransferase III/MGAT3 protein
Tyr31-Val533, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Enzymes
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
42-54 & 64-70 kDa, reducing conditions
Read Publication using
7359-GT in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Assay Procedure
  • Buffer A: 25 mM MES, 5 mM MnCl2, pH 6.5
  • Buffer B: 100 mM Tris, 5 mM CaCl2, pH 7.5
  • Recombinant Human N-Acetylglucosaminyltransferase III/MGAT3 (rhMGAT3) (Catalog # 7359-GT)
  • Donor Substrate: UDP-GlcNAc (Sigma, Catalog # U4375), 50 mM stock in 50% ethanol
  • Acceptor Substrate: Biantennary N-Linked Core Pentasaccharide (V-Labs, Catalog # M592), 10 mM stock in deionized water
  • Glycosyltransferase Activity Kit (Catalog # EA001)
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute 1 mM Phosphate Standard provided by the Glycosyltransferase Kit by adding 40 µL of the 1 mM Phosphate Standard to 360 µL of Buffer A for a 100 µM stock.
  2. Prepare standard curve by performing six one-half serial dilutions of the 100 µM Phosphate stock in Buffer A. The standard curve has a range of 0.078 to 5 nmol per well.
  3. Dilute rhMGAT3 to 10 µg/mL in Buffer A.
  4. Dilute Donor Substrate to 1 mM in Buffer A.
  5. Dilute Acceptor Substrate to 1 mM in Buffer A.
  6. Prepare Substrate Mixture by combining 120 µL of 1 mM Donor Substrate, 120 µL of 1 mM Acceptor Substrate, and 60 µL of Buffer A.
  7. Load 50 µL of each dilution of the standard curve into a plate. Include a curve blank containing 50 μL of Buffer A.
  8. Load 25 µL of the 10 µg/mL rhMGAT3 into the plate. Include a Control containing 25 µL of Buffer A.
  9. Start the reaction by adding 25 µL of Substrate Mixture to the wells, excluding the standard curve.
  10. Cover the plate with a plate sealer and incubate at 37 °C for 20 minutes.
  11. Dilute Coupling Phosphatase 1 to 2 μg/mL in Buffer B.
  12. Add 50 µL of 2 µg/mL Coupling Phosphatase 1 to reaction wells and blanks, excluding the standard curve. Also, add 50 µL of Buffer B to the wells containing the standard curve.  Mix and incubate for 10 minutes at room temperature.
  13. Add 30 µL of the Malachite Green Reagent A to all wells.
  14. Add 50 µL of deionized water to all wells.
  15. Add 30 µL of the Malachite Green Reagent B to all wells. Mix and incubate for 20 minutes at room temperature.
  16. Read plate at 620 nm (absorbance) in endpoint mode.
  17. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Phosphate released* (nmol) x (1000 pmol/nmol)
Incubation time (min) x amount of enzyme (µg)

     *Derived from the phosphate standard curve using linear or 4-parameter fitting and adjusted for Control.

Per Well:
  • rhMGAT-3: 0.25 µg
  • Coupling Phosphatase 1: 0.1 µg
  • Donor Substrate: 10 nmol
  • Acceptor Substrate: 10 nmol


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human N-Acetylglucosaminyltransferase 3/MGAT3 CF

  • beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
  • EC
  • FLJ43371
  • GGNT3
  • GlcNAc-T III
  • GNT3
  • GNT-IIIMGC142278
  • mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
  • MGAT3
  • MGC141943
  • N-acetylglucosaminyltransferase III
  • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III


Mannosylglycoprotein N-acetyl-glucosaminyltransferase 3 (MGAT3), also known as GnT-III, regulates the branching of N-glycans. By transferring a GlcNAc residue to the beta -linked mannose of the trimannosyl core of N-linked oligosaccharides MGAT3 produces a bisecting GlcNAc structure (1). Bisecting GlcNAc is involved in a number of biological events, including the suppression of metastasis of cancer cells through modification on integrins (2, 3, 4) and the reduction of hepatitis B virus replication in hepatoma cells (5). The effect of the bisecting GlcNAc on a cancer cell counteracts that of the beta 1,6-GlcNAc created by MGAT5 (4, 6). Although MGAT3 and MGAT5 are functionally related, there is no significant homology at their primary sequences. In addition, bisecting GlcNAc on human Ig antibodies enhances their effector functions (7). Transgenic mice over expressing MGAT3 had aberrant glycosylation on apolipoprotein B and developed fatty livers (8). The enzymatic activity of recombinant human MGAT3 was determined using a phosphatase-coupled glycosyltransferase assay (9).
  1. Ihara, Y. et al. (1993) J. Biochem. 113:692.
  2. Yoshimura, M. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8754.
  3. Isaji T, et al. (2004) J. Biol. Chem. 279:19747.
  4. Sato, Y. et al. (2009) J. Biol. Chem. 284:11873.
  5. Miyoshi, E. et al. (1995) J. Biol. Chem. 270:28311.
  6. Gu, J. and Taniguchi, N. (2008) Cell Adh. Migr. 2:243.
  7. Stanley P. (2002) Biochim. Biophys. Acta. 1573:363.
  8. Ihara, Y. et al. (1998) Proc. Natl. Acad. Sci. USA 95:2526.
  9. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
Species: Hu
Applications: WB, IHC
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow, KO
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
Species: Hu
Applications: WB, Simple Western, IHC, IP
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: EnzAct.

Filter By Application
All Applications
Filter By Species
All Species

Reviews for N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT) (0)

There are no reviews for N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT)

Discover related pathways, diseases and genes to N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT)

Discover more about diseases related to N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT).

Pathways for N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT)

View related products by pathway.

PTMs for N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT)

Learn more about PTMs related to N-Acetylglucosaminyltransferase III/MGAT3 (7359-GT).

Blogs on N-Acetylglucosaminyltransferase III/MGAT3

There are no specific blogs for N-Acetylglucosaminyltransferase III/MGAT3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Albumin Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human N-Acetylglucosaminyltransferase 3/MGAT3 CF and receive a gift card or discount.


Gene Symbol MGAT3