FPR1 Antibody - BSA Free

Western Blot: FPR1 Antibody [NB100-56473] - Analysis of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using this antibody at 1 ug/ml.

Simple Western: FPR1 Antibody [NB100-56473] - Shows a specific band for FPR1 in 0.5 mg/ml of Mouse Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

• Reactivity: Hu, Mu, Pm
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FPR1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide corresponding to amino acids 176-190 of human FPR1 (CTFNFSPWTNDPKER) was used as the immunogen
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FPR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western 100 ug/ml
  • Western Blot
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Mouse Liver lysate 0.5 mg/mL, separated by Size, antibody dilution of 100 ug/mL
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FPR1 Antibody - BSA Free

• fMet-Leu-Phe receptor
• fMLF-R
• fMLP receptor
• FMLP
• formyl peptide receptor 1
• FPR1
• FPRfMet-Leu-Phe receptor
• N-formyl peptide receptor
• N-formylpeptide chemoattractant receptor

Background

FPR1 is a member of chemoattractant/chemokine GPCRs expressed mainly on leukocytes, neutrophils and monocytes. FPR1 is a seven transmembrane pertussis toxin-sensitive GPCR and binds to formylated peptides generated by bacteria resulting in leukocyte trafficking to the site of inflammation. Ligand binding to FPR results in activation of multiple effectors including phospholipase C leading to calcium mobilization and PKC activation. Other implications of FPR signaling include polarization of actin cytoskeleton, activation of various integrins, increased cell migration, phagocytosis, degranulation and release of reactive oxygen intermediates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FPR1 Antibody (NB100-56473)(3)

Publications using NB100-56473

• YE Kim, SY Ahn, DK Sung, YS Chang, WS Park Mesenchymal Stem Cells and Formyl Peptide Receptor 2 Activity in Hyperoxia-Induced Lung Injury in Newborn Mice International Journal of Molecular Sciences, 2022-09-13;23(18):. 2022-09-13 [PMID: 36142517] (Western Blot)
• Zayas JP Molecular Dissection of Impaired Infection Control in Diabetic Wound Thesis 2020-01-01
• Roy R, Zayas J, Singh SK et al. Overriding impaired FPR chemotaxis signaling in diabetic neutrophil stimulates infection control in murine diabetic wound eLife 2022-02-03 [PMID: 35112667] (WB)

Bioinformatics

• Gene Symbol: FPR1
• Entrez:
  • 2357(Human)
• Uniprot:
  • P21462(Human)